Robot Automation of Sampling and Sample Management during Cultivation of Mammalian Cells in Pilot Scale
In mammalian cell culture technology the last key issue for a completely automated process is the taking, analysing and storing of a sample from the bioreactor. It was necessary to develop a new device which fulfils the need to be steam sterilizable for an application in industrial production environment. The status of the culture is derived from the results of the analysed parameters of these samples. The normal procedure includes determination of the total and viable cell densities in relation to the media parameters (glucose, lactic acid, ammonia). The cell densities are retrieved by counting a stained cell sample. A cell free aliquot is stored in a freezer for further analyses (e.g. amino acids and product). Information for optimisation of feed and harvest strategies can be retrieved from the analysed parameters additionally.
KeywordsPilot Scale Sample Management Viable Cell Density Laboratory Device Automation Strategy
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