Development of Immunomicroscopic Methods for Bioremediation

  • K. C. Ruel
  • J.-P. Joseleau
Chapter
Part of the NATO Science Series book series (NAIV, volume 19)

Abstract

In nature, wood can be completely degraded by lignolytic fungi. Basidiomycetous white-rot fungi are particularly efficient wood degraders because of their ability to completely mineralize lignin [1,2]. The enzymes produced by white-rot fungi are directed against the two types of macromolecules that make up the walls of wood cells, Polysaccharides and polyphenols.. These enzymes consist of Polysaccharide hydrolases that break down cellulose and hemicelluloses, and of a series of oxidizing enzymes that are able to attack and depolymerize lignins. The latter group consists of a variety of extra-cellular enzymes among which peroxidases — principally lignin peroxidase (LiP) and manganese-dependent peroxidase (MnP) — together with phenol oxidases laccase (Lac), aryl-alcohol oxidase (AAO) and other nonphenol oxidases, contribute to the highly complex mechanisms leading to lignin depolymerization, aromatic rings opening and demethylation [3, 4, 5]. An essential step in the enzymic attack of lignin is the formation of aryl cation radicals originating from both phenolic and nonphenolic aromatic rings [1, 4, 6]. This is the typical mode of action of LiP which uses hydrogen peroxide to generate aryl cation radicals. The other major peroxidase, particularly efficient in lignin oxidation, is manganese peroxidase which oxidizes manganese ions from Mn+2 to Mn+3. These ions that have a short life-time, are stabilized by organic acids, mostly dicarboxylic acids, and are thought to diffuse in the tight lignocellulosic network of the wood cell walls [7]. Another component of the enzyme complex participating in lignin oxidation is laccase, a phenol oxidase which seems now to be rather widespread amont the most efficient wood-degrading white-rot fungi [8].

Keywords

Wheat Straw Lignin Peroxidase Phanerochaete Chrysosporium Ligninolytic Enzyme Manganese Peroxidase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. 1.
    Kirk, T.K.. and Farrell, R.L. (1987) Enzymatic combustion: the microbial degradation of lignin, Annu. Rev. Microbiol. 41, 465–505CrossRefGoogle Scholar
  2. 2.
    Waldner, R., Leisola, M.S.A., and Fletcher, W. (1988) Comparison of lignolytic activities of selected white-rot fungi. Appl. Microbiol. Biotechno. 29, 400–407.CrossRefGoogle Scholar
  3. 3.
    Wariishi, H., Valli, K., and Gold, M.H. (1991) In vitro depolymerization of lignin by manganese peroxidase of Phanerochaete chrysosporium, Biochem. Biophys. Res. Comm. 176, 269–275.CrossRefGoogle Scholar
  4. 4.
    Eriksson, K.-E.L., Blanchette, R.A. and Ander, P. (1990) Microbial and enzymatic degradation of wood components, Springer-Verlag Berlin, Heidelberg.CrossRefGoogle Scholar
  5. 5.
    Hatakka, A. (1994) Lignin-modifying enzymes from selected white-rot fungi: production and role in lignin degradation. FEMS Microbiol. Rev. 13, 125–135.CrossRefGoogle Scholar
  6. 6.
    Schoemaker, H.E., Lundell, T., Hatakka, A. and Piontek, K. (1994) The oxidation of veratryl alcohol, dimeric lignin models and lignin by lignin peroxidase: the redox cycle revised. FEMS Microbiol. Rev. 13, 314–321.CrossRefGoogle Scholar
  7. 7.
    Daniel, G. (1994) Use of electron microscopy for aiding our understanding of wood biodegradation FEMS Microbiol. Rev. 13, 199–234.CrossRefGoogle Scholar
  8. 8.
    Bourbonnais, R. and Paice, M.G. (1990) Oxidation of non-phenolic substrates, an expanded role for laccase in lignin biodegradation. FEBS Lett. 267, 99–102.CrossRefGoogle Scholar
  9. 9.
    Munoz, C., Guiller, F., Martinez, A.T. and Martinez, M.J. (1997) Laccase isoenzymes of Pleurotus eryngii: characterization, catalytic properties, and participation in activation of molecular oxygen and Mn+2 oxidation. Appl. Environ. Microbiol. 63, 2166–2174.Google Scholar
  10. 10.
    Eggert, C., Temp, U., Dean, J.F.D., and Eriksson, K.E.L. (1996) A fungal metabolite mediates degradation of non phenolic lignin structures and synthetic lignin by laccase. FEBS Lett. 391, 144–148CrossRefGoogle Scholar
  11. 11.
    Eriksson, K.E., Habu, N., and Samajima, M. (1993) Recent advance in fungal cellobiose oxidoreductases. Enzyme Microb. Technol. 15, 1002–1008.CrossRefGoogle Scholar
  12. 12.
    Hammel, K.E. (1989) OrganopoUutant degradation by ligninolytic fungi. Enzyme Microb. Technol. 11, 776–777.CrossRefGoogle Scholar
  13. 13.
    Joshi, D.K., and Gold, M.H. (1994) Oxidation of di-benzo-p-dioxin by lignin peroxidase from the basidiamycete Phanerochaete chrysosporium. Biochemistry 33, 10969–10975.CrossRefGoogle Scholar
  14. 14.
    Sack, U., Hofrichter, M., and Fritsche, W. (1997) Degradation of polycyclic aromatic hydrocarbons by manganese peroxidase of Nematoloma frowardii. FEMS Microbiol. Lett. 152, 227–234CrossRefGoogle Scholar
  15. 15.
    Novotny, C., Erbanova, P., Sasek, V., Kubatova, A., Cajthaml, T., Lang, E., Krahl, J. and Zadrazil, F. (1999) Extracellular oxidative enzyme production and PAH removal in soil by exploratory mycelium of white-rot fungi. Biodegradation 10, 159–168.CrossRefGoogle Scholar
  16. 16.
    Boyle, C.D. (1995) Development of a practical method for inducing white-rot fungi to grow into and degrade organo-pollutants in soil. Can. J. Microbiol. 41, 345–353.CrossRefGoogle Scholar
  17. 17.
    Laine, M.M., and Jorgensen, K.S. (1996) Straw compost and bioremediatiated soil as inocula for bioremediation of chlorophenol-contaminated soil, Appl, Environ. Microbiol. 62, 1507–1513.Google Scholar
  18. 18.
    Lang, E., Nerud, F., and Zadrazil, F. (1998) Production of ligninolytic enzymes by Pleurotus sp. and Dichomitus squalens in soil and lignocellulose substrate as influenced by soil microorganisms, FEMS Microbiol. Lett. 167, 239–244.CrossRefGoogle Scholar
  19. 19.
    Lang, E., Nerud, F., Novotna, E., Zadrazil, F., and Martens, R. (1996) Production of ligninolytic exoenzymes and pyrene mineralization by Pleurotus sp. in lignocellulose substrate, Folia Microbiol. 41, 489–493.CrossRefGoogle Scholar
  20. 20.
    Vares, T., Kalsi, M., and Hatakka, A. (1995) Lignin peroxidases, manganese-peroxidases, and other ligninolytic enzymes produced by Phlebia radiata during solid-stage fermentation of wheat straw. Appl. Environ. Microbiol. 61, 3515–3520.Google Scholar
  21. 21.
    Ruel, K., Faix, O., and Joseleau, J-P. (1994) New immunogold probes for studying the distribution of the different lignin types during plant cell wall biogenesis. J. Trace Microprobe Techniques 12, 247–265.Google Scholar
  22. 22.
    Joseleau, J.-P., and Ruel, K. (1997) Study of lignification by noninvasive techniques in growing maize internodes. An investigation by FTIR, CP/MAS 13C NMR and immunocytochemical transmission electron microscopy, Plant Physiol. 114, 1123–1133.CrossRefGoogle Scholar
  23. 23.
    Sarkanen, K.V. (1971) Lignin precursors and their polymerization. Lignins: occurrence, formation, structure and reactions, Sarkanen KV. and Ludwig G.H., eds., Wiley-Interscience, New York, pp. 95–163.Google Scholar
  24. 24.
    Ruel, K., Ambert, K. and Joseleau, J.-P. (1994) Influence of the enzyme equipment of white-rot fungi on the patterns of wood degradation. FEMS Microbiol. Rev. 13, 241–254.CrossRefGoogle Scholar
  25. 25.
    Ruel, K., Burlat, V. and Joseleau, J-P. (1999) Relationship between ultrastructural topochemistry of lignin and wood properties. IAWA J. 20, 2: 203–211.Google Scholar
  26. 26.
    Ruel, K., Barnoud, F. and Eriksson, K.E. (1981) Micromorphological and ultrastructural aspects of spruce wood degradation by wild-type Sporotrichum pulverulentum and its cellulase-less mutant cel 44. Holzforschung, 35, 157–171.CrossRefGoogle Scholar
  27. 27.
    Bes, B., Pettersson, B., Lennholm, H., Iversen, T. and Eriksson, K.E. (1987) Synthesis, structure and enzyme degradation of an extracellular glucan produced in nitrogen-starved cultures of the white rot fungus Phanerochaete chrysosporium, Appl. Biochem. Biotechnol. 9, 310–318.Google Scholar
  28. 28.
    Ruel, K. and Joseleau, J.-P. (1991) Involvement of an extracellular glucan sheath during degradation of Populus wood by Phanerochaete chrysosporium. Appl. Environ. Microbiol. 57, 374–384.Google Scholar
  29. 29.
    Srebotnik, E., Messner, K. and Foisner, R. (1988) Penetrability of white rot degraded pine wood by the lignin peroxidase of Phanerochaete chrysosporium. Appl. Environ. Microbiol. 54, 2608–2614.Google Scholar
  30. 30.
    Daniel, G., Petterson, B., Nilsson, T. and Volc, J. (1990) Use of immunogold cytochemistry to detect Mn(II)-dependent and lignin-peroxidases in wood degraded by the white rot fungi Phanerochaete chrysosporium and Lentinula edodes. Can. J. Bot. 68, 920–933.CrossRefGoogle Scholar
  31. 31.
    Joseleau, J-P. and Ruel, K. (1992) Ultrastructural examination of lignin and Polysaccharide degradation in wood by white rot fungi. In: Biotechnology in Pulp and Paper Industry. M. Kuwahara, M. Shimada eds. Uni Publishers CO, LTD, Ohyn Bidg, 2-6-8 Kayaba-cho, Nihonbashi, Tokyo, Japan, part II, 195–202.Google Scholar
  32. 32.
    Saloheimo, M., Niku-Paavola, M-L. and Knowles, J.K.C. (1991) Isolation and structural analysis of the laccase gene from the lignin-degrading fungus Phlebia radiata. J. Gen. Microbiol. 137, 1537–1544.Google Scholar
  33. 33.
    Ruel, K., Zhang, H., Niku-Paavola, M-L; Saloheimo, M., Moukha, S. and Joseleau, J-P (1999) In situ Hybridization and Immunocytochemistry in Electron Microscopy to Study the Expression of Ligninolytic Enzymes in Fungi Growing in Wood. Proceedings from the 10 th International Symposium on Wood and Pulping Chemistry, Yokohama, Japan, Vol. I, 534–539.Google Scholar
  34. 34.
    Janse, B.J.H., Gaskell, J., Akhtar, M. and Cullen, D. (1998) Expression of Phanerochaete chrysosporium genes encoding lignin peroxidases, manganese peroxidases, and glyoxal oxidase in wood. Appl. Environ. Microbiol. 64, 3536–3538.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2003

Authors and Affiliations

  • K. C. Ruel
    • 1
  • J.-P. Joseleau
    • 1
  1. 1.Centre de Recherches sur les Macromolécules VégétalesCNRSGrenoble, cedex 9France

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