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Cell Culture Techniques for the Identification of Enteric Viruses — Present Possibilities

  • J. C. Bridger
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Part of the Current Topics in Veterinary Medicine and Animal Science book series (CTVM, volume 13)

Abstract

The current techniques available for the detection of transmissible gastroenteritis virus, bovine and porcine rotaviruses, bovine enteric Coronavirus, bovine astrovirus and bovine parvovirus are reviewed. Although, initially, the enteric pathogens were difficult to establish in culture regularly, cell culture methods are currently available for identification of several of them. Some of the viruses can be detected by serial passage with cytopathology but many techniques rely on the production of antigens which are identified by immunofluorescent staining. The techniques which have been used to detect serological differences between the viruses and to detect viral antibody are reviewed and some of the advantages and disadvantages of cell culture systems are discussed.

Keywords

Cytopathic Effect Serial Passage Enteric Virus Cell Culture Technique Neonatal Calf 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© ECSC, EEC, EAEC, Brussels-Luxembourg 1981

Authors and Affiliations

  • J. C. Bridger
    • 1
  1. 1.A.R.C. Institute for Research on Animal DiseasesBerkshireUK

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