Nucleic Acids: The Vectors of Life pp 305-315 | Cite as
Fluorescence Decay Studies of Peptide-Nucleic Acid Complexes
Abstract
Fluorescence decay studies of two tryptophan-containing peptides and one tyrosine-containing peptide reveal the existence of at least two conformers for each peptide. When these peptides bind to poly(U), denatured or native DNA changes are observed in both the lifetimes and the respective contributions of the two components. A model is proposed in which each peptide conformer forms two types of complexes, one involving only electrostatic interactions, the second one involving both electrostatic and stacking interactions. Moreover energy transfer from tyrosine to nucleic acid bases is shown to play an important role in the quenching of the fluorescence of tyrosine-containing peptides bound to nucleic acids.
Keywords
Fluorescence Lifetime Fluorescence Quantum Yield Fluorescence Decay Nucleic Acid Basis Exponential Decay FunctionPreview
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