The Alkaline Earth Metals—Biological Applications

  • Torbjörn Drakenberg
  • Sture Forsén
Chapter
Part of the NATO ASI Series book series (ASIC, volume 103)

Abstract

Both 25Mg and 43Ca are nuclei with low receptivity due to low magnetogyric ratios and, especially for 43Ca, low natural abundance. It is therefore not surprising that the interest in these nuclei in biological studies by NMR is of quite recent date, despite the well documented importance of these nuclei in biological systems (1). The first 25Mg and 43Ca NMR studies with some biological implications appeared in 1969 (2,3); however, not until 1978 was the first study of metal binding to proteins published (4). Even though subsequently it has been made clear that both 25Mg and 43Ca NMR can be very useful tools in biological studies, very few groups are active in this area, as can be judged from the literature. This is most probably, at least for 43Ca, due to the high cost for enriched samples. In this chapter we will show, with a few examples, how 25Mg and 43Ca can be used to obtain information about the metal binding to proteins and other biologically interesting molecules. Some of this information is hard, if not impossible, to get with other methods, e.g., metal exchange rates, whereas other problems can often be better solved by other methods, e.g., binding constants.

Keywords

Exchange Rate Biological Application Quadrupole Coupling Constant Calcium Binding Site Weak Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© D. Reidel Publishing Company 1983

Authors and Affiliations

  • Torbjörn Drakenberg
    • 1
  • Sture Forsén
    • 1
  1. 1.Physical Chemistry 2, Chemical CenterUniversity of LundLund 7Sweden

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