Sym Plasmids in RhizobiumJaponicum

  • Kim S. Engwall
  • Nancy DuTeau
  • Alan G. Atherly
Part of the Advances in Agricultural Biotechnology book series (AABI, volume 4)

Abstract

Rhizobiumjaponicum strains PRC191 and PRC193 are fast-growing strains that were isolated in the People’s Republic of China (H. Keyser et al., 1982). Recently it was shown that these strains have nif and nod genes on large plasmids (P. Masterson et al., 1982, and unpublished).We have introduced Tn5 into the Sym plasmids of PRC191 and PRC 193. The Sym plasmids are not self-transmissible. Therefore, we introduced a helper plasmid, pRL180 (P.J.J. Hooykaas et al., 1982), into these strains to promote plasmid mobilization to other Rhizobia and Agrobacterium strains.

Keywords

Nitrogen Fixation Helper Plasmid Junk Publisher Large Plasmid Symbiotic Property 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Hooykaas PJJ, den Dulk-Ras H, and Schilperoort RA (1982) Plasmid 8, 94–95.PubMedCrossRefGoogle Scholar
  2. Keyser HH, Bohlool BB, Hu TS, and Weber DF (1982) Science 215, 1631–1632.PubMedCrossRefGoogle Scholar
  3. Masterson RV, Russell PR, and Atherly AG (1982) 3. Bacteriol. 152, 928–931.Google Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers, The Hague and Pudoc, Wageningen 1984

Authors and Affiliations

  • Kim S. Engwall
    • 1
  • Nancy DuTeau
    • 1
  • Alan G. Atherly
    • 1
  1. 1.Dept. of GeneticsIowa State UniersityAmesUSA

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