Membrane biosynthesis

  • Edith Sim
Chapter

Abstract

Early investigations of mechanisms of membrane synthesis were concerned with resolving the question of whether ‘new membrane’ was synthesized at focal points or whether the various components were added independently to an existing framework. One method of investigating membrane synthesis is to use a synchronous cell population. For rapidly dividing bacteria and for investigating virus replication in infected cells this is easily arranged by starting at a low inoculum or level of infection. For eucaryotic cells, which divide more slowly, it is possible to separate cells at different stages of the cell cycle according to density. Such a separated cell population is shown in Fig. 5.1. As the cell progresses through the cycle, the surface becomes more and more convoluted by the appearance of microvilli [1]. To determine if ‘new membrane’ is being synthesized intact, it is necessary to investigate at the molecular level and to compare the synthesis of the individual components.

Keywords

Signal Sequence Coated Vesicle Semliki Forest Virus Membrane Synthesis Eucaryotic Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Additional Reading

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Copyright information

© E. Sim 1982

Authors and Affiliations

  • Edith Sim
    • 1
  1. 1.Department of BiochemistryUniversity of OxfordUK

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