Metabolism of polyamines in NaCl-resistant cell lines from Nicotiana sylvestris
The polyamine titers in three cell lines of Nicotiana sylvestris were compared: Type 1, rapidly adapting to NaCl: Type 2, constantly resistant to NaCl; Type 3, a salt-sensitive wild strain. During short-term cultivation in MS medium in the presence of 170mM NaCl (1 passage, 14 d) the changes in polyamine titer in cell suspensions of type 1 (in a slightly adapted state) and non-adapted wild strain (type 3) showed a considerable increase in spermidine and spermine and a decrease in putrescine. After prolonged adaptation to NaCl (20 passages) the putrescine content in the cells of type 1 and of type 2 was increased at the expense of the polyamines. This suggests that the pattern of polyamine titer varies under short- and long-term adaptation to NaCl. The inverse ratio between growth processes and changes in polyamine and proline level indicates that polyamines fulfil primarily a protective and osmorepulatory function in plant cells under NaCl sttess.
Key wordsSalt-resistant cell lines polyamines proline growth
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- 1.Bagni N, Cremonini M (1973) Induction and repression of polyamine synthesis in Oenothera biennis by different salts. Inform Botan Italiano 5: 103–104Google Scholar
- 5.Friedman R, Altman A, Bachrach U (1983) Polyamines involvement in plant response to salt stress. Supplement to Plant Physiol 72: 137Google Scholar
- 6.Gilston AW, Flores HE, Young ND (1983) Putrescine formation by arginine decarboxylase: a stress response in cereal leaves. Supplement to Plant Physiol 72: 105Google Scholar
- 7.Kiryan IG, Shevyakova NI, (1983) The lack of regulation of methionine biosynthesis in salt-resistant cell line of Nicotina sylvestris. Abstracts of IV All-Union Conference ‘Plant Tissue Culture and Biotechnology’. Kishinev 162Google Scholar
- 8.Kiryan IG, Shevyakova NI (1984) Proline accumulation pathways in NaC1- resistant line of Nicotiana sylvestris cells. Fiziol Rast (Moscow) 31: 712–720Google Scholar
- 9.Oshmarina VI, Shamina ZB (1982) Cell culture of Nicotiana sylvestris: Growth characteristics and the selective system for isolating salt-tolerant species. Fiziol Rast (Moscow) 29: 161–167Google Scholar
- 10.Oshmarina VI, Shamina ZB, Shevyakova NI (1983) Characteristics of Nicotiana sylvestris cell lines resistant to NaC1 and ethionine. Fiziol Rast (Moscow) 30: 527–536Google Scholar
- 11.Prikhod’ko LS, Klyshev LK (1964) Nitrogen metabolism of pea shoots at different substrate salinity. Tr Inst Botan Akad Nauk KSSR 20: 166–182Google Scholar
- 12.Shevyakova NI, Arutyunova NV, Strogonov BP (1981) Disturbance of arginine and putrescine metabolism in cotton leaves in the presence of excessive Na2SO4. Fiziol Rast (Moscow) 28: 594–600Google Scholar
- 13.Shevyakova NI, Kiryan IG, Strogonov BP (1984) The increased rate ot spermidine biosynthesis in NaC1-resistant cell line in Nicotiana sylvestris. Fiziol Rast (Moscow) 31: 810–816Google Scholar
- 14.Strogonov BP (1962) Physiological basis of salt tolerance of plants. Pub Acad Sci USSR MoscowGoogle Scholar