Metabolism of polyamines in NaCl-resistant cell lines from Nicotiana sylvestris

  • N. I. Shevyakova
  • B. P. Strogonov
  • I. G. Kiryan
Part of the Advances in Agricultural Biotechnology book series (AABI, volume 18)


The polyamine titers in three cell lines of Nicotiana sylvestris were compared: Type 1, rapidly adapting to NaCl: Type 2, constantly resistant to NaCl; Type 3, a salt-sensitive wild strain. During short-term cultivation in MS medium in the presence of 170mM NaCl (1 passage, 14 d) the changes in polyamine titer in cell suspensions of type 1 (in a slightly adapted state) and non-adapted wild strain (type 3) showed a considerable increase in spermidine and spermine and a decrease in putrescine. After prolonged adaptation to NaCl (20 passages) the putrescine content in the cells of type 1 and of type 2 was increased at the expense of the polyamines. This suggests that the pattern of polyamine titer varies under short- and long-term adaptation to NaCl. The inverse ratio between growth processes and changes in polyamine and proline level indicates that polyamines fulfil primarily a protective and osmorepulatory function in plant cells under NaCl sttess.

Key words

Salt-resistant cell lines polyamines proline growth 


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  1. 1.
    Bagni N, Cremonini M (1973) Induction and repression of polyamine synthesis in Oenothera biennis by different salts. Inform Botan Italiano 5: 103–104Google Scholar
  2. 2.
    Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant and Soil 39: 205–207CrossRefGoogle Scholar
  3. 3.
    Flores HE, Galston AW (1982) Analysis of polyamines in higher plants by high performance liquid chromatography. Plant Physiol 69: 701–706PubMedCrossRefGoogle Scholar
  4. 4.
    Flores HE, Galston AW (1982) Polyamines and plant stress: Activation of putrescine biosynthesis by osmotic shock. Science 217: 1259–1260PubMedCrossRefGoogle Scholar
  5. 5.
    Friedman R, Altman A, Bachrach U (1983) Polyamines involvement in plant response to salt stress. Supplement to Plant Physiol 72: 137Google Scholar
  6. 6.
    Gilston AW, Flores HE, Young ND (1983) Putrescine formation by arginine decarboxylase: a stress response in cereal leaves. Supplement to Plant Physiol 72: 105Google Scholar
  7. 7.
    Kiryan IG, Shevyakova NI, (1983) The lack of regulation of methionine biosynthesis in salt-resistant cell line of Nicotina sylvestris. Abstracts of IV All-Union Conference ‘Plant Tissue Culture and Biotechnology’. Kishinev 162Google Scholar
  8. 8.
    Kiryan IG, Shevyakova NI (1984) Proline accumulation pathways in NaC1- resistant line of Nicotiana sylvestris cells. Fiziol Rast (Moscow) 31: 712–720Google Scholar
  9. 9.
    Oshmarina VI, Shamina ZB (1982) Cell culture of Nicotiana sylvestris: Growth characteristics and the selective system for isolating salt-tolerant species. Fiziol Rast (Moscow) 29: 161–167Google Scholar
  10. 10.
    Oshmarina VI, Shamina ZB, Shevyakova NI (1983) Characteristics of Nicotiana sylvestris cell lines resistant to NaC1 and ethionine. Fiziol Rast (Moscow) 30: 527–536Google Scholar
  11. 11.
    Prikhod’ko LS, Klyshev LK (1964) Nitrogen metabolism of pea shoots at different substrate salinity. Tr Inst Botan Akad Nauk KSSR 20: 166–182Google Scholar
  12. 12.
    Shevyakova NI, Arutyunova NV, Strogonov BP (1981) Disturbance of arginine and putrescine metabolism in cotton leaves in the presence of excessive Na2SO4. Fiziol Rast (Moscow) 28: 594–600Google Scholar
  13. 13.
    Shevyakova NI, Kiryan IG, Strogonov BP (1984) The increased rate ot spermidine biosynthesis in NaC1-resistant cell line in Nicotiana sylvestris. Fiziol Rast (Moscow) 31: 810–816Google Scholar
  14. 14.
    Strogonov BP (1962) Physiological basis of salt tolerance of plants. Pub Acad Sci USSR MoscowGoogle Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers, Dordrecht 1985

Authors and Affiliations

  • N. I. Shevyakova
    • 1
  • B. P. Strogonov
    • 1
  • I. G. Kiryan
    • 1
  1. 1.Timiriazev Institute of Plant Physiology USSR Academy of SciencesMoscowUSSR

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