New Developments in Biosciences: Their Implications for Laboratory Animal Science pp 431-436 | Cite as
Assessment of Discomfort Induced by Orbital Puncture in Rats
Abstract
Possible discomfort induced by orbital puncture in rats was assessed by assigning scores per punctured and non-punctured eye of each animal to the following parameters: photophobia, enophthalmos, discharge, superficial damage, internal damage and reduced withdrawal upon approaching with a cotton tip. Scoring was performed independently and blind (the rats were punctured either at the left or right eye) by 4 assessors. Out of the 6 parameters only enophthalmos tended to occur more frequently in punctured than control eyes. There was a considerable between-assessor variation in the assignment of scores to enophthalmos. It remains to be established whether enophthalmos is associated with discomfort or pain in rats.
Keywords
Cholesterol Metabolism Internal Damage Diet Code Superficial Damage Animal TechnicianPreview
Unable to display preview. Download preview PDF.
References
- 1.Beynen AC, Baumans V, Bertens APMG, Havenaar R, Hesp APM, Van Zutphen LFM. Assessment of discomfort in gallstone-bearing mice: a practical example of the problems encountered in an attempt to recognize discomfort in laboratory animals. Lab Anim 1987; 21:35–42.PubMedCrossRefGoogle Scholar
- 2.Beynen AC, Lemmens AG. Dietary glycine and cholesterol metabolism in rats. Z Ernährungswiss 1987; in press.Google Scholar