Preliminary observations on psychrotrophic and psychrophilic, heterotrophic bacteria from antarctic water samples

  • T. A. McMeekin
Conference paper
Part of the Developments in Hydrobiology book series (DIHY, volume 34)

Abstract

Bacteria isolated from antarctic sea and lake water samples were examined for growth at 5°C and 25°C. Of 250 isolates, 249 were psychrotrophic and one was psychrophilic. The effect of temperature on the growth rate of the latter (strain 755) and a typical psychrotroph (strain 203A) was described by the square root of the growth rate constant. TMIN values calculated from this model confirmed the strains as psychrophile and psychrotroph.

Identification of 93 isolates showed the majority to be Pseudomonas (82%), with Flavobacterium and Moraxella representing 13% of the isolates. The remainder were coryneforms and yeasts. Isolate 611 was studied in detail because of its unusual morphology. This strain has features in common with the genera Spirosoma, Runella and Flectobacillus, but cannot be assigned with certainty. It may represent a new taxon of curved, gram negative bacteria

Key words

antarctic bacteria curved bacteria temperature relations identification 
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References

  1. Baross, J. A. & J. Liston, 1970. Occurrence of Vibrio parahaemolyticus and related hemolytic vibrios in marine environments of Washington State. Appl. Microbiol. 20: 179–187.PubMedGoogle Scholar
  2. Baross, J. A. & R. Y. Morita, 1978. Microbial life at low temperatures: Ecological aspects. In D. J. Kushner (ed.), Microbial Life in Extreme Environments. Academic Press, N.Y. & Lond. 10–57.Google Scholar
  3. Cameron, R. E., F. A. Morelli & R. M. Johnson, 1972. Bacterial species in soil and air of the Antarctic Continent. Antarct. J.U.S. 7: 187–189.Google Scholar
  4. Holmes, B., R. J. Owen & T. A. McMeekin, 1984. The genus Flavobacterium. In N. R. Kreig (ed.), Bergey’s Manual of Systematic Bacteriology, I. Williams & Wilkins, Baltimore: 353–361.Google Scholar
  5. Larkin, J. M. & R. Borrall, 1984. Spirosomaceae. In N. R. Kreig (ed.), Bergey’s Manual of Systematic Bacteriology, I. Williams & Wilkins, Baltimore: 125–132.Google Scholar
  6. Lewis, T. E., 1984. Microflora of coralline algae. B.Sc.(Hons.) Thesis. University of Tasmania: 36–38.Google Scholar
  7. Liston, J., 1951. The occurrence and distribution of bacterial types on flatfish. J. gen. Microbiol. 16: 205–216.Google Scholar
  8. Liston, J. & R. R. Colwell, 1963. Host and habitat relationships of marine commensal bacteria. In C. H. Oppenheimer (ed.) Symposium on Marine Microbiology. C. C. Thomas, Springifield, Illinois: 611–624.Google Scholar
  9. Morita, R. Y., 1975. Psychrophilic bacteria. Bacteriol. Rev. 39: 144–167.PubMedGoogle Scholar
  10. Novitsky, J. A. & R. Y. Morita, 1976. Morphological characterisation of small cells resulting from nutrient starvation of a psychrophilic marine Vibrio. Appl. Environ. Microbiol. 32: 617–622.PubMedGoogle Scholar
  11. Novitsky, J. A. & R. Y. Morita, 1977. Survival of a psychrophilic marine Vibrio under long-term nutrient starvation. Appl. Environ. Microbiol. 33: 635–641.PubMedGoogle Scholar
  12. Novitsky, J. A. & R. Y. Morita, 1978. Possible strategy for the survival of marine bacteria under starvation conditions. Mar. Biol. 48: 289–295.CrossRefGoogle Scholar
  13. Postgate, J. R. & J. R. Hunter, 1962. The survival of starved bacteria. J. gen. Microbiol. 29: 233–263.PubMedGoogle Scholar
  14. Ratkowsky, D. A., J. Olley, T. A. McMeekin & A. Ball, 1982. Relationship between temperature and growth rate of bacterial cultures. J. Bacteriol. 149: 1–5.PubMedGoogle Scholar
  15. Ratkowsky, D. A., R. K. Lowry, T. A. Meekin, A. N. Stokes & R. E. Chandler, 1983. Model for bacterial culture growth rate throughout the entire biokinetic temperature range. J. Bacteriol. 154: 1222–1226.PubMedGoogle Scholar
  16. Shewan, J. M., G. Hobbs & W. Hodgkiss, 1960. A determinative scheme for the identification of certain genera of bacteria with special reference to the Pseudomonadaceae. J. appl. Bacteriol. 23: 379–390.Google Scholar
  17. Shewan, J. M. & T. A. McMeekin, 1983. Taxonomy (and ecology) of Flavobacterium and related genera. Ann. Rev. Microbiol. 37: 233–252.CrossRefGoogle Scholar
  18. Stevenson, L. H., 1978. A case for bacterial dormancy in aquatic systems. Microb. Ecol. 4: 127–133.CrossRefGoogle Scholar
  19. Thomas, C. J. & T. A. McMeekin, 1980. Contamination of broiler carcass skin during commercial processing procedures: an electron microscopic study. Appl. Environ. Microbiol. 40: 133–144.PubMedGoogle Scholar
  20. Wiebe, W. J. & C. W. Hendricks, 1974. Distribution of heterotrophic bacteria in a transect of the Antarctic Ocean. In R. R. Colwell & R. Y. Morita (eds) Effect of the Ocean Environment on Microbial Activities. University Park Press, Baltimore: 524–535.Google Scholar
  21. Xu, H. S., N. Roberts, F. L. Singleton, R. W. Attwell, D. J. Grimes & R. R. Colwell, 1982. Survival and viability of non-culturable Escherichia coli and Vibrio cholerae in the estuarine and marine environment. Microb. Ecol. 8: 313–323CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • T. A. McMeekin
    • 1
  1. 1.Department of Agricultural ScienceUniversity of TasmaniaHobartAustralia

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