Immobilized Biospecific Proteins in Analytical Clinical Chemistry

  • A. Roda
  • S. Girotti
  • G. Carrea
  • P. Simoni
  • M. A. Angellotti
  • R. Rizzoli
Chapter
Part of the NATO ASI Series book series (ASIC, volume 226)

Abstract

Immobilized proteins are widely used in the clinical laboratory, e.g., immobilized enzymes in assay for analytes such as glucose, uric acid and immobilized antigen and antibodies in immunoassay. In the enzymatic analysis the use of immobilized enzymes make these reagents recoverable and thus reusable and in most cases increases their stability and catalytic activity. In conjuction with bioluminescent enzymes (firefly and bacterial luciferases) we set up high sensitive flow methods based on the use of nylon tube coil as solid support.

All the NAD(P)/NAD(P)H dependent dehydrogenases (bacterial luciferase), ATP dependent enzymes (firefly luciferase) and oxidases producing H2O2 (peroxidase) can be immobilized and a large variety of analytes have been sensitively measured.

In the immunoassay the immobilization of an antibody facilitate the separation of the protein from other components in solution i.e. For example an antigen-antibody complex. The combination of immobilized antibody with luminescent detection of the antigen-antibody reaction gave very specific and sensitive immunoluminescent metodologies.

Keywords

Bile Acid Immobilize Enzyme Immobilize Antibody Oxalate Oxidase Maximum Binding Capacity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© D. Reidel Publishing Company 1988

Authors and Affiliations

  • A. Roda
    • 1
  • S. Girotti
    • 2
  • G. Carrea
    • 3
  • P. Simoni
    • 2
  • M. A. Angellotti
    • 2
  • R. Rizzoli
    • 2
  1. 1.Institute of Analytical ChemistryUniversity of MessinaItaly
  2. 2.Institute of Chemical ScienceUniversity of BolognaItaly
  3. 3.Institute of Chemistry of HormonesCNRMilanItaly

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