Advertisement

Complete Fluorescence Emission Spectra Determined During the Induction Kinetic Using a Diode-Array Detector

  • Claus Buschmann
  • Hartmut K. Lichtenthaler

Abstract

By using a diode-array detector it has become possible for the first time to measure with one leaf sample complete fluorescence emission spectra during the fast rise and the slow decline of the Kautsky induction kinetic. Thus fast spectral changes in the millisecond range can be detected. Changes in pigment-protein complexes during the first seconds of illumination of an etiolated leaf can be measured at room temperature. During the Kautsky induction kinetic of photosynthetically active green leaves, the maxima of the emission spectrum does not change. However, compared to the long wavelength signal at the maximum at about 735 nm, the short wavelength signal at the maximum at about 690 nm in general increases faster during the fast rise and decreases faster during the slow decline. This is demonstrated for greening and fully green maize leaves. The spectral changes can be expressed by the ratio between the fluorescence signals at the two emission maxima (F690/F735) calculated from the stored spectra taken during the fast rise and the slow decrease of the induction kinetic. The general validity of this ratio change during the Kautsky kinetic is demonstrated for leaves of a variety of different plant species each with a high and a low chlorophyll content.

Key Words

Chlorophyll fluorescence diode-array detector forest decline Kautsky effect fluorometer photosynthesis ratio F690/F735 Rfd-values 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Buschmann C, 1981. The characterization of the developing photosynthetic apparatus in greening barley leaves by means of (slow) fluorescence kinetic measurements. In: Photosynthesis, Vol 5, Akoyunoglou G ed, pp 417–426. Balaban International Science Services, Philadelphia.Google Scholar
  2. Buschmann C and Schrey H, 1981. Fluorescence induction kinetics of green and etiolated leaves by recording the complete in-vivo emission spectra. Photosynthesis Research 1: 233–241.CrossRefPubMedGoogle Scholar
  3. Buschmann C and Sironval C, 1979. Influence of kinetin on protochlorophyll-(ide) accumulation and on the Shibata shift in Raphanus seedlings. Planta 139: 127–132.CrossRefGoogle Scholar
  4. Buschmann C and Sironval C, 1984. Fluorescence emission spectra of etiolated leaves measured at 296 and 77 K during the first seconds of continuous illumination. In: Protochlorophyllide reduction and greening, Sironval C and Brouers M, eds, pp 139–148.CrossRefGoogle Scholar
  5. Jouy M and Sironval C, 1979. Quenching of the fluorescence emitted by P695–682 at room temperature in etiolated illuminated leaves. Planta 147: 127–133.CrossRefPubMedGoogle Scholar
  6. Kocsanyi L, Haitz M and Lichtenthaler HK, 1988. Measurement of laser-induced chlorophyll fluorescence kinetics using a fast acoustooptic device. In: Applications of chlorophyll fluorescence. Lichtenthaler HK ed, pp 92–100, Kluwer Academic Press, Dordrecht.Google Scholar
  7. Lichtenthaler HK and Buschmann C, 1987. Chlorophyll fluorescence spectra of green bean leaves. J Plant Physiol 129: 137–147.CrossRefGoogle Scholar
  8. Lichtenthaler HK and Buschmann C, 1988. Changes in the chlorophyll fluorescence spectra during the Kautsky induction kinetics. In: Proceedings of the 4th International Colloquium on Spectral Signatures of Objects in Remote Sensing, Aussois, pp. 245–250,ESA Publications Division, Noordwijk.Google Scholar
  9. Lichtenthaler HK, Buschmann C, Rinderle U and Schmuck G, 1986. Application of chlorophyll fluorescence in ecophysiology. Radiation and Environmental Biophysics 25: 297–308.CrossRefPubMedGoogle Scholar
  10. Rinderle U and Lichtenthaler HK, 1988. The chlorophyll fluorescence ratio F690/F735 as a possible stress indicator. In: Applications of chlorophyll fluorescence, Lichtenthaler HK ed, pp 176–183, Kluwer Academic Press, Dordrecht.Google Scholar
  11. Schreiber U, Fink R and Vidaver W, 1977. Fluorescence induction in whole leaves: Differentiation between the two leaf sides and adaptation to different light regimes. Planta 133: 121–129.CrossRefPubMedGoogle Scholar
  12. Sironval C and Brouers M, 1984. Protochlorophyllide reduction and greening. Martinus Nijhoff/Dr W Junk Publishers, The Hague.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • Claus Buschmann
    • 1
  • Hartmut K. Lichtenthaler
    • 1
  1. 1.Botanisches Institut II (Plant Physiology and Plant Biochemistry)University of KarlsruheKarlsruheGermany

Personalised recommendations