The most important classical limnological parameters are mentioned and the possibility to measure corresponding values by chlorophyll fluorescence are reviewed. These are mainly the chlorophyll content and the photosynthetic turnover which allows estimates of primary production. In contrast to forestry or agricultural applications hydrobiological work faces mixtures of many species in the sample and smaller and very varying chlorophyll concentrations. Since fluorescence methods are quick and rather easy to be mechanised they allow to obtain the spacial and temporal distribution of phytoplancton. The influence of poisons on a test culture can be checked by fluorescence too. Different colour classes (e.g. bluegreen, or green algae) can be identified to some extend by their excitation or fluorescence spectra.

Key words

Chlorophyll fluorescence hydrobiology limnology eutrophication poisioning oceanography O2-depletion monitoring of algae chlorophyll content electron transport chain blocker chlorophyll extraction primary production quench effect delayed fluorescence colour classes 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Author Group ESA (European Space Agency) — Study, 1986. The use of chlorophyll fluorescence measurements from space for separating constituents of seawater.GKSS, Geesthacht.Google Scholar
  2. Beneke G, Falke W, Schmidt Chr, 1982. Use of algal fluorescence in an automated monitoring system. Bull Env Tox 28: 385CrossRefGoogle Scholar
  3. Christoffers D, Ernst DEW, 1983. The in-vivo fluorescence of Clorella fusca as a biological test for the inhibition of photosynthesis. Tox Env Chem 7: 61–71.CrossRefGoogle Scholar
  4. Ernst D, 1986. Comments on fluorometric chlorophyll determination in the field. Arch Hydrobiol 107: 521–527.Google Scholar
  5. Ernst D. The Kinetics of Chlorophyll Extraction from Phytoplancton and a quick extraction method. Arch Hydrobiol Erg Limnol (in press).Google Scholar
  6. Güther S, 1987. A quick method for extractive chlorophyll determinations in the fluorometer. Arch Hydrobiol Erg Limnol Beih 29: 93–98.Google Scholar
  7. Krause G H, Weis E, 1984. Chlorophyl fluorescence as a tool in plant physiology. II Interpretation of fluorescence signals.Phot Syn Res 5: 139–157.Google Scholar
  8. Krause H, Dirnhofer P, Gerhardt V, 1987: The application of delayed fluorescence. Arch Hydrobiol Erg Limnol Beih 29: 55–62.Google Scholar
  9. Margalef R, 1967. Concepts relative to the organization of plancton. Oceanog Mar Biol Ann Rev 5: 257–289.Google Scholar
  10. Noack U, 1983. Untersuchungen über Chlorophyll a-Gehalt, Chlorophyll-Fluoreszenz und weitere limnologische Parameter am Phytoplankton des Dümmers. Diplomarbeit, Inst.f.Biophysik,Uni Hannover.Google Scholar
  11. Noack U, 1983. Bestimmung der Phytoplankton-Biomasse über Chlorophyll-a-Gehalt und Chlorophyllfluoreszenlz am Dümmer. Mitt Nieders Wasser-unterungsuchsamt Hildesheim.Google Scholar
  12. Nusch A, 1980. Comparison of different methods for chlorophyll and phaeo-pigment determination. Arch Hydrobiol Beih 14: 14–36.Google Scholar
  13. Nusch A. In vivo fluorescence — a biomass — or a bioactivity indicator? Arch Hydrobiol (in press)Google Scholar
  14. Rai H, Marker A, 1982: The measurement of photosynthetic pigments in fresh-waters and standarization of methods. Arch Hydrobiol Erg Limnol Beih 16.Google Scholar
  15. Reckel S, 1984. Vergleichende Untersuchungen von Primärproduktionsmessungen und Fluoreszenzverhalten von Algen in Versuchsteichen.StaatsexamensarbeitGoogle Scholar
  16. Renger G, Schreiber U, 1986. Practical applications of fluorometric methods to algae and higher plant research. In: Light emission by plants and bacteria. Govindjee, Amesz J, Fork D C (eds) Acad Press, New York.Google Scholar
  17. Samuelsson G, Öquist G, Halldal P R, 1978. The variable chlorophyll a fluorescence as a measure of photosynthetic capacity in algae. Mitt Int Verein Limnol 21: 207–215.Google Scholar
  18. Yentsch Ch, Yentsch Cl, 1979. Fluorescence spectral signatures: The characterization of phytoplancton populations by the use of excitation and emission spectra. J Marine Res 37: 3.Google Scholar

Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • Dieter E. W. Ernst
    • 1
  1. 1.Inst. f. BiophysikHannoverGermany

Personalised recommendations