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Fiber — Optic Detection of Chlorophyll Fluorescence

  • Herbert Schneckenburger
  • Joachim Bader

Abstract

A fiber-optic system for detection of in vivo fluorescence was developed. Exciting argon laser light and fluorescence are transmitted through the same fiber using appropriate micro-optic devices (dichroic mirrors, filters, selfoc lenses), an optional spectral analyzer and an avalanche photodetector. This system allows for optimum flexibility, remote sensing and detection of small areas in the submillimeter range of biological samples. Preliminary measurements include the induction and decrease of chlorophyll fluorescence during light exposure of intact spruce needles and needles submitted to water stress.

Key words

Fiber-optic sensor fluorescence detection 

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References

  1. Bader J, 1988. Aufbau und Optimierung eines faseroptischen Sensors zur Messung schwacher Fluoreszenzsignale. Diplomarbeit, Fachhochschule Aalen.Google Scholar
  2. Krause H, Gerhardt V and Gebhardt W, 1984. Verzögerte Fluoreszenz lebender Pflanzenzellen. Phys. unserer Zeit 15: 182–190.CrossRefGoogle Scholar
  3. Lichtenthaler HK, Buschmann C, Rinderle U and Schmuck G, 1986. Application of Chlorophyll fluorescence in ecophysiology. Radiat. Environ. Biophys. 25: 297–308.Google Scholar
  4. Scheper I and Schügerl K, 1986. Characterization of bioreactors by in-situ fluorometry. J. Biotechnol. 3: 224–229.Google Scholar
  5. Schneckenburger H, Reuter BW and Schoberth SM, 1985. Fluorescence techniques in biotechnology. Trends Biotechnol. 3: 257–261.CrossRefGoogle Scholar
  6. Schneckenburger H and Frenz M, 1986. Time-resolved fluorescence of conifers exposed to environmental pollutants. Radiat. Environ. Biophys. 25: 289–295.CrossRefPubMedGoogle Scholar
  7. Schneckenburger H, Frenz M, Tsuchiya Y, Denzer U and Schleinkofer L, 1987. Picosecond fluorescence microscopy for measuring chlorophyll and porphyrin components in conifers and cultured cells. Lasers Life Sci. 1: 299–307.Google Scholar
  8. Schreiber U, 1986. Differenzierung zwischen verschiedenen Typen der Fluoreszenzlöschung und Bestimmung der relativen Elektronen-Transportrate in vivo mit Hilfe der Sättigungspuls-Methode. Symp. Chlorophyll Fluorescence in Ecology, Hannover (FRG).Google Scholar
  9. Schulze E, Pätzold G, Röther-Steinke M and Ernst D, 1983. Chlorophyllfluoreszenz zur Früherkennung von Photosythesebeeinträchtigungen an Coniferen. Forstarchiv 54: 229–232.Google Scholar
  10. Wolfbeis OS, 1986. Analytical chemistry with optical sensors. Fresenius Z. Anal. Chem. 325: 387–392.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • Herbert Schneckenburger
    • 1
    • 2
  • Joachim Bader
    • 1
  1. 1.Fachhochschule AalenAalenGermany
  2. 2.Institut für Lasertechnologien in der Medizin an der Universität UlmUlmGermany

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