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Assessment of UV-B Stress by Chlorophyll Fluorescence Analysis

  • M. Tevini
  • P. Grusemann
  • G. Fieser

Abstract

UV-B stress was evaluated by measuring chlorophyll fluorescence induction kinetics with the PAM-fluorometer. Commonly used parameters such as Fo, Fm, Fv, Fp, qQ and qE are compared in sunflower seedlings grown under enhanced artificial or solar UV B radiation. It was shown that Fo increased whereas Fm and Fv decreased under enhanced artificial UV-B. Photochemical phase Fo-I1 decreased at a similar degree as “thermal” phase I1-I2. The half life of fluorescence dark decay after a single turnover flash was shorter under UV-B stress. In the steady state photochemical quenching qQ decreased with UV-B exposure whereas energy dependent quenching qE increased. Similar results were obtained under solar radiation at a Mediterranian latitude.

Key Words

Ozone depletion UV-B radiation stress sunflower Chlorophyll fluorescence q-analysis photosystem II 

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References

  1. BJöRN LO, BORNMAN JF and OLSSON E, 1986. Effects of ultraviolet radiation on fluorescence induction kinetics in isolated thylakoids and intact leaves. In: Stratospheric Ozone Reduction, Solar Ultraviolet Radiation and Plant Life (Worrest AC and Caldwell MM., ed.). NATO ASI Series G: Ecological Sciences, Vol. 8, Springer BerlinGoogle Scholar
  2. CROFTS AR and WRAIGHT CA, 1983. The electrochemical domain of photosynthesis. Biochim. Biophys. Acta 726, 149–185CrossRefGoogle Scholar
  3. DEMMIG B, WINTER K, KRüGER A and CZYGAN FC, 1987. Photoinhibition and zeaxanthin formation in intact leaves. Plant Physiol., 84, 218–224 Fabian P, 1988. Antarktisches Ozonloch: Indizien weisen auf Umweltverschmutzung, Phys. Bl. 44, Nr. 1, 2–7Google Scholar
  4. IWANZIK W, 1983. Wirkung von UV-Strahlung auf das Wachstum von Keimlingen und die Zusammensetzung und photosynthetische Aktivität von Blättern und isolierten Chloroplasten. Karlsruher Beiträge zur Entwicklungsphysiologie der Pflanzen (Hrsg.: M. Tevini), Nr.5, Universität KarlsruheGoogle Scholar
  5. IWANZIK W, TEVINI M, DOHNT G, VOSS M, WEISS W, GRäBER P and RENGER G., 1983. Action of UV-B radiation on pho-tosynthetic primary reactions in spinach chloroplasts. Physiol. Plant. 58, 401–407CrossRefGoogle Scholar
  6. MOLINA MJ and ROWLAND FS, 1974. Stratospheric sink for chlorofluoromethanes: Chlorine atom catalysed destruction of ozone. Nature 249, 810–812CrossRefGoogle Scholar
  7. NEUBAUER C and SCHREIBER U, 1987. The polyphasic rise of chlorophyll fluorescence upon onset of strong continuous illumination: I. Saturation characteristics and partial control by the photosystem II acceptor side. Z. Naturforsch. 42c, 1246–1254CrossRefGoogle Scholar
  8. RENGER G, VOSS M, GRäBER P and SCHULZ A, 1986. Effect of UV irradiation on different partial reactions of the primary processes of photosynthesis. In: Stratospheric Ozone Reduction, Solar Ultraviolet Radiation and Plant Life (Worrest AC and Caldwell MM., ed.). NATO ASI Series G: Ecological Sciences, Vol. 8, Springer BerlinGoogle Scholar
  9. SCHREIBER U, 1986. Detection of rapid induction kinetics with a new type of high-frequency modulated chlorophyll fluorometer. Photosynth. Res. 9, 261–272CrossRefPubMedGoogle Scholar
  10. SCHREIBER U and NEUBAUER C, 1987. The Polyphasic Rise of Chlorophyll Fluorescence upon Onset of Strong Continuous Illumination: I. Partial Control by the Photosystem II Donor Side and Possible Ways of Interpretation. Z. Naturforsch. 42 c, 1255–1264CrossRefGoogle Scholar
  11. SCHREIBER U, SCHLIWA U and BILGER W, 1986. Continous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth. Res. 10, 51–62CrossRefPubMedGoogle Scholar
  12. TEVINI M and IWANZIK W, 1983. Inhibition of photosynthetic activity by UV-B radiation in radish seedlings. Physiol. Plant. 58, 395–400CrossRefGoogle Scholar
  13. TEVINI M and PFISTER K, 1985. Inhibition of photosystem II by UV-B radiation. Z. Naturforsch. 40c, 129–133CrossRefGoogle Scholar
  14. VU CV, ALLEN LH and GARRARD LA, 1984. Effects of Enhanced UV-B Radiation (280–320 nm) on Ribulose-1,5-Bisphosphat Carboxylase in Pea and Soybean. Environm. Experim. Bot. 24, No 2, 131–143CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • M. Tevini
    • 1
  • P. Grusemann
    • 1
  • G. Fieser
    • 1
  1. 1.Botanisches Institut IIUniversität Karlsruhe (TH)KarlsruheGermany

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