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Winter Stress And Chlorophyll Fluorescence in Norway Spruce (Picea abies, L., Karst.)

  • H. R. Bolhàr-Nordenkampf
  • E. Lechner

Abstract

Winter stress induces loss of photosynthetic capacity in at least two ways: 1.) At freezing temperatures below -4° C the photosynthetic membranes were disintegrated. 2.) Chilling temperatures enhance the sensitivity for photoinhibition. Fast kinetics of chlorophyll fluorescence induction (FV/Fm = Fm-F0/Fm; Fm = P) provide adequate means to assess the photosynthetic capacity of a plant.

Samples from the experimental garden (Vienna) and from three sites in the alpine region (Zillertal) were measured with a microscopic fluorimeter and additionally with a portable fluorimeter. Freezing temperatures induce a marked loss of FV/Fm independently of incident light-intensities. FV/Fm also declined if chilling temperatures are combined with high light-intensities. Chilling temperatures are combined with low light had no photoinhibitory effect. Therefore, shaded and snow covered branches are protected against light stree.

Chlorophyll flourescence is a sensitive tool to detect stress phenomena as well as a measuring device which is manageable even under unfavourable climatic conditions.

Key words

fluorescence induction winter stress photoinhibition Picea abies 

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References

  1. Bolhàr-Nordenkampf, HR and Lechner, E, 1988: Temperature and light dependent modifications of chlorophyll fluorescence kinetics in spruce needles during winter. Photosyn Res, in press.Google Scholar
  2. Bongi, G and Long, SP, 1987: Light-dependent damage of photosynthesis in olive leaves during chilling and high light treatment. Plant, Cell Environ 10: 241–249.Google Scholar
  3. Buschmann, C, 1986: Fluoreszenz- und Wärmeabstrahlung bei Pflanzen. Naturwissenschaften 73: 691–699.CrossRefGoogle Scholar
  4. Butler, WL and Kitajima, M, 1975: A tripartite model for chloroplast fluorescence. In: Avron, M. Proceedings of the 3rd international congress on photosynthesis. Elsevier, Amsterdam.Google Scholar
  5. Ireland, CR, Telfer, A, Covello, PS, Baker, NR and Barber, J., 1988: Studies on the limitations to photosynthesis in leaves of the atrazine resistant mutant of Senecio vulgaris L. Planta 173: 459–467.CrossRefPubMedGoogle Scholar
  6. Krause, GH and Somersalo, S, 1988: Fluorescence as a tool in photosynthesis research: application in studies of photoinhibition, cold acclimation and freezing stress. Proc R Soc Lond, in press.Google Scholar
  7. Lichtenthaler, HK, Buschmann, C, Rinderle, U and Schmuck, G, 1986: Application of chlorophyll fluorescence in ecophysiology. Radiat Environ Biophys 25: 297–308.CrossRefPubMedGoogle Scholar
  8. Martin, B, Martensson, O and Öquist, G, 1978: Effects of frost hardening and dehardening on photosynthetic electron transport and fluorescence properties in isolated chloroplasts of Pinus silvestris. Physiol Plant 43: 297–305.CrossRefGoogle Scholar
  9. Öquist, 1983: Effects of low temperature on photosynthesis. Plant Cell Environ 6: 281–300.Google Scholar
  10. Renger, G and Schreiber, U, 1986: Practical applications of fluorimetric methods to algae and higher plant research. In: Govindjee, Amesz, A and Fork, DC. Light emission by plants and bacteria. Academic Press, Orlando.Google Scholar
  11. Sakai, A and Larcher, W, 1987: Mechanisms of frost survival. In: Mechanisms of frost survival. Responses and adaptation to freezing stress. Springer Verlag, Berlin.CrossRefGoogle Scholar
  12. Senser, M and Beck, E, 1979: Kälteresistenz der Fichte. II Einfluß von Photoperiode und Temperatur auf die Struktur und photochemischen Reaktionen von Chloroplasten. Ber Dt Bot Ges, Bd. 92: 243–259.Google Scholar
  13. Strand, M. and Lundmark, T, 1987: Effects of low night temperature and light on chlorophyll fluorescence of field-grown seedlings of Scots pine. Tree Physiol 3: 211–224.CrossRefPubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • H. R. Bolhàr-Nordenkampf
  • E. Lechner
    • 1
  1. 1.Dept. for Horticultural Plant Physiology, Institute for Plant PhysiologyUniversity of ViennaViennaAustria

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