Measurement of the Laser-Induced Chlorophyll Fluorescence Kinetics Using a Fast Acoustooptic Device

  • László Kocsanyi
  • Michael Haitz
  • Hartmut K. Lichtenthaler


A laser induced computer-aided fluorometer system (LICAF) is presented which permits monitoring of the chlorophyll-fluorescence induction kinetics at two different wavelengths (690 and 730 nm regions) excited by a HeNe-laser. The exciting laser beam is controlled by a fast acoustooptic device. The rising time of the illumination is shorter than 200 ns. The data acquisition rate of the LICAF is at maximum 2000 data/sec, at each of the two wavelength regions.

The newly developed system permits measurement in 5 us periods, eyery 500 us of the complete chlorophyll fluorescence induction kinetics (fast and slow components of the Kautsky effect) of predarkened leaves. The ratio of the fluorescence in the 690 and 730 nm region (F690/F730) is calculated and plotted together with the induction kinetics. Examples of the application of the LICAF in the stress detection in plants e.g. water stress and herbicide (diuron) treatment is demonstrated.

Key Words

acoustooptic device Bragg cell chlorophyll fluorescence fluorescence induction kinetics fluorescence ratio F690/F730 Kautsky effect 


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Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • László Kocsanyi
    • 1
  • Michael Haitz
    • 2
  • Hartmut K. Lichtenthaler
    • 2
  1. 1.Department of Atomic PhysicsTU BudapestBudapestHungary
  2. 2.Botanisches Institut II (Plant Physiology and Plant Biochemistry)University of KarlsruheKarlsruheGermany

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