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Measurement of the Laser-Induced Chlorophyll Fluorescence Kinetics Using a Fast Acoustooptic Device

  • László Kocsanyi
  • Michael Haitz
  • Hartmut K. Lichtenthaler

Abstract

A laser induced computer-aided fluorometer system (LICAF) is presented which permits monitoring of the chlorophyll-fluorescence induction kinetics at two different wavelengths (690 and 730 nm regions) excited by a HeNe-laser. The exciting laser beam is controlled by a fast acoustooptic device. The rising time of the illumination is shorter than 200 ns. The data acquisition rate of the LICAF is at maximum 2000 data/sec, at each of the two wavelength regions.

The newly developed system permits measurement in 5 us periods, eyery 500 us of the complete chlorophyll fluorescence induction kinetics (fast and slow components of the Kautsky effect) of predarkened leaves. The ratio of the fluorescence in the 690 and 730 nm region (F690/F730) is calculated and plotted together with the induction kinetics. Examples of the application of the LICAF in the stress detection in plants e.g. water stress and herbicide (diuron) treatment is demonstrated.

Key Words

acoustooptic device Bragg cell chlorophyll fluorescence fluorescence induction kinetics fluorescence ratio F690/F730 Kautsky effect 

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References

  1. Jakab L, Kocsanyi L, Giber J, 1985. Bandwith limiting factors of the acoustic transducers of TeO2 acoustooptic devices. In: Proceed. Symposium on Electronics Technology Budapest, Vol. II, pp. 198–201.Google Scholar
  2. Krause GH and Weis E, 1984. Chlorophyll fluorescence as a tool in plant physioloqy II. Interpretation of fluorescence signals. Photosynth. Res. 5: 139–157.CrossRefGoogle Scholar
  3. Larcher W and Cernusca A, 1985. Microcomputer-gesteuerte mobile Anlage zum fluorometrisehen Nachweis von Photosynthesestörungen. Sitzungsber. Österr. Akad. Wissensch., Math.-naturw. Kl. Abt. I 194, pp. 45–64, Springer Verlag, Wien.Google Scholar
  4. Lichtenthaler HK, 1984. Influence of environmental factors on composition and function of the photosynthetic apparatus. In: Advances in Photosynthesis Research, Sybesma C ed., Vol. IV, pp. 241–244, Martinus Nijhoff/Dr. W. Junk Publisher, The Hague.CrossRefGoogle Scholar
  5. Lichtenthaler HK, 1986. Laser-induced chlorophyll fluorescence of living plants. In: Proceed. Remote Sensing Symp. IGARSS Zürich Vol. III, pp. 1587–1590, ESA Publications Division, Noordwijk.Google Scholar
  6. Lichtenthaler HK, 1987. Chlorophyll fluorescence signatures of leaves during the autumnal chlorophyll breakdown. J. Plant Physiol. 131: 101–110.CrossRefGoogle Scholar
  7. Lichtenthaler HK and Buschmann C, 1987. Chlorophyll fluorescence spectra of green bean leaves. J. Plant Physiol. 129: 137–147.CrossRefGoogle Scholar
  8. Lichtenthaler HK and Rinderle U, 1988. Role of chlorophyll fluorescence in the detection of stress conditions in plants. CRC Critical Reviews in Analytical Chemistry 19, Suppl. I, S29-S85.Google Scholar
  9. Lichtenthaler HK, Buschmann C, Rinderle U und Schmuck G, 1986. Application of Chlorophyll fluorescence in ecophysiology. Radiation Environmental Biophysics 25: 297–308.CrossRefPubMedGoogle Scholar
  10. Neubauer C and Schreiber U, 1986. The polyphasic rise of chlorophyll fluorescence upon onset of strong continuous illumination. I. Saturation characteristics and partial control by the photosystem II acceptor side. Z. Naturforsch. 42c: 1246–1254.Google Scholar
  11. Strasser R, Schwarz B and Bucher J, 1987. Simultane Messung der Chlorophyll Fluoreszenz-Kinetik bei verschiedenen Wellenlängen als rasches Verfahren zur Frühdiagnose von Immissionsbelastungen an Waldbäumen. Ozoneinwirkung auf Buchen und Pappeln. Europ. J. Forest Pathology 17: 149–157.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • László Kocsanyi
    • 1
  • Michael Haitz
    • 2
  • Hartmut K. Lichtenthaler
    • 2
  1. 1.Department of Atomic PhysicsTU BudapestBudapestHungary
  2. 2.Botanisches Institut II (Plant Physiology and Plant Biochemistry)University of KarlsruheKarlsruheGermany

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