Progress in Plant Protoplast Research pp 109-110 | Cite as
A Protoplast System for Populus Which will Support Genetic Engineering Research of Tree Species
Chapter
Abstract
The use of genetic engineering is an appropriate technology for the improvement of tree crops because breeding by classical methods has been difficult. Of the 2 main avenues for plant transformation, the Agrobacterium vector system has been successfully used to insert an economically important gene into a commercial forest tree (Populus) [1]. The protoplast-based transformation systems (eg. fusion, microinjection, direct gene transfer), however, have not been tested in woody plants because of the lack of suitable methods to recover whole plants from protoplasts of most tree genotypes.
Keywords
Poplar Hybrid Leaf Protoplast Direct Gene Transfer Viable Protoplast Main Avenue
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
- 1.Fillatti, J. J., J. Sellmer, B. McCown, B. Haissig, and L. Comai. 1987. Agrobacterium mediated transformation and regeneration of Populus. Mol. Gen. Genet. 206:192–199.CrossRefGoogle Scholar
- 2.Russell, J.A. and B.H. McCown. 1986. Techniques for enhanced release of leaf protoplasts in Populus. Plant Cell Rep. 5:284–287.CrossRefGoogle Scholar
- 3.Russell, J.A. and B.H. McCown. 1986. Culture and regeneration of Populus leaf protoplasts isolated from non-seedling tissue. Plant Sci. 46:133–142.CrossRefGoogle Scholar
- 4.Russell, J.A. and B.H. McCown. Recovery of plants from leaf protoplasts of hybrid-poplar and aspen clones. Plant Cell Rep. (In Press).Google Scholar
Copyright information
© Kluwer Academic Publishers 1988