Selecton for Resistance to Phoma Lingam Using Pycnospore Infections of Plants in-Vivo and Sirodesmin PL as Selective Agent in-Vitro
The aim of the study was to find resistant accessions within Brassicaceae to Phoma lingam and to evaluate whether resistance could be expressed on protoplast or cellular level in presence of the toxin, sirodesmin PL. A gene transfer system was designed, for transfer of genes for resistance to B. napus via protoplast fusions, using the toxin as a selective agent in the screening for resistant hybrid cells.
KeywordsCell Aggregate Selective Agent Protoplast Fusion Donor Material Greenhouse Grown Plant
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- 1.Delwiche, P. A. 1980. Genetic aspects of blackleg (Leptosphaeria maculans) resistance in rapeseed (Brasssica napus) Ph.D. Thesis, University of Wisconsin, Madison, 144pp.Google Scholar
- 5.Sjödin, C, Nyhammar, T. and K. Glimelius 1987. Effects of toxic metabolites produced by Phoma lingam (Tode ex Fr) Desm. on protoplasts, cells and plants of hosts and non—hosts to the pathogen. J. Phys. and Mol. Pl. Path. (In press).Google Scholar
- 7.Sundberg, E., Landgren, M. and K. Glimelius 1987. Fertility and chromosome stability in Brassica napus synthesized by protoplast fusion. Theor. Appl. Genet. (In press).Google Scholar
- 8.Williams, P.A. 1985. Crucifer Genetics Cooperative (CrGC) Resource Book, Dept. of Plant Pathology, Univ. of Wisconsin, Madison, USA.Google Scholar