Quantitative applications of the resolution of enantiomers by capillary electrophoresis

  • K. D. Altria
Chapter

Abstract

The separation and quantitation of enantiomers are important applications of capillary electrophoresis. Other chapters cover the background to the separation selectivities that can be obtained in free solution capillary electrophoresis (FSCE) and micellar electrokinetic capillary chromatography (MECC) using a variety of chiral selectors such as cyclodextrins (CDs), bile salts and crown ethers. This chapter covers the validation and quantitative application of chiral CE methods. Table 24.1 covers the majority of quantitative applications with selected details of selectivity modes, performance and application. The validation criteria applied to chiral CE methods are similar to those employed when assessing HPLC (high performance liquid chromatography) methods. Standard criteria assessed include linearity, repeatability, reproducibility, precision and accuracy. HPLC and CE may be employed in tandem to confirm accuracy. The majority of quantitative reports have focused on the analysis of chiral pharmaceuticals and agrochemicals. Application areas include enantiomeric purity testing of single enantiomer compounds, reaction rate monitoring, stability testing and enantiomeric separation of drugs in clinical samples.

Keywords

Capillary Electrophoresis Crown Ether Capillary Zone Electrophoresis Chiral Selector Chiral Separation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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© Chapman & Hall 1997

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  • K. D. Altria

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