Epidermal dimensions at the ultrastructural level
In most structural studies the tissue under examination is killed by fixation. This effectively reduces the opportunity to examine changes in time and we are left with three spatial dimensions. It is important to appreciate that in most instances the three-dimensional configuration of a tissue is seen as a two-dimensional section. When attempting to quantify dimensions this two-dimensional representation of structure plays an important role in influencing the values derived and affecting the sample size required, which is enormous in the case of ultrastructural observations. To understand the importance of dimensions, it is necessary to study the means of achieving such quantities and the potential errors in their interpretation. To examine one ultrathin section at 10 000 x magnification and extrapolate this sole observation to the whole tissue is akin to examining one blue wall in one room of a multi-storey block and concluding that the whole building is probably blue.
KeywordsStratum Corneum Lichen Planus Ultrastructural Level Epidermolysis Bullosa Ultrastructural Observation
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- 1.Aherne, W.A. and Dunhill, M.S. (1982). Morphometry. (London: Edward Arnold)Google Scholar
- 2.Weibel, E.R. (1979). Stereological Methods. Vol. 1. Practical methods for biological morphometry. (New York: Academic Press)Google Scholar
- 3.Williams, M.A. (1977). Quantitative methods in biology. In Glauert, A.M. (ed.) Practical Methods in Electron Microscopy. Vol. 6. (Amsterdam: North Holland Publishing Co.)Google Scholar
- 9.Mandelbrot, B.B. (1977). Fractals: Form, Chance, and Dimension. (San Francisco: Freeman and Co.)Google Scholar
- 10.Holmes, A.H. (1921). Petrographic Methods and Calculations. (London: Murby)Google Scholar
- 11.Chalkey, H.W. (1943). Method for quantitative morphologic analysis of tissue. J. Natl. Cancer Inst. 4, 47Google Scholar
- 13.Merz, W.A. (1986). Streckenmessung an gerichtenen strukturen im Mikroskop und ihre Andwendung zur Bestimmung von Oberflachen-Volumen-relationen im Knochengewbe. Mikroskopie, 22, 132Google Scholar
- 17.Lindberg, M. (1982). Variation in epidermal structure as a function of different fixation methods. A stereological and morphological study. Ph.D dissertation, Dept. of Medical Biophysics, Karolinska Institute, Stockholm, SwedenGoogle Scholar
- 18.Lindberg, M., Johannesson, A. and Forslind, B. (1982). The effect of occlusive treatment on human skin: an electron microscopic study on epidermal morphology as affected by occlusion and dansyl chloride. Dermato-Venerol. (Stockh.), 62, 1Google Scholar