Ethylene Production in Relation to Rose Micropropagation through Axillary Budding

  • Thomas Gaspar
  • Claire Kevers
  • Hélène Bouillenne
  • Yves Maziere
  • Jean-Pierre Barbe
Part of the Advances in Agricultural Biotechnology book series (AABI, volume 26)


Cultured decapitated shoots (double axes on socle) from Madame Georges Delbard rose proliferated multiple shoots on a modified Murashige and Skoog medium supplemented with IBA (0.1 mg 1−1) and BAP (1.5 mg 1−1) in a multiplication cycle of 21 days.A peak of ethylene production by the cultured rose explants correlated with the initiation (days 4–8) of lateral shoot outgrowth from basal axillary buds. Such a profile of ethylene production was not recorded on a rooting medium (IBA alone). The peak of ethylene production on the multiplication medium could be amplified by incorporating in the medium either ACC(the ethylene production enhancement was out of proportion with the slight stimulation of budding), or IBA and BAP at a 1: 1 ratio (both at 0.75 or 1.5 mg 1−1, which did not allow any budding). Ethylene production was slown down on the contrary and the peak suppressed by the addition of AVG and CoCl2 (AOA and AIB were toxic) without interference with the proliferation of axillary buds. The results thus provide evidence that there is no function al relationship between axillary budding and ethylene biosynthesis.

Key words

axillary budding ethylene production in vitro micropropagation rose vegetative multiplication 



1-aminocyclopropane-l-carboxylic acid


1-aminoisobutyric acid


aminooxyacetic acid acid




gibberellic acid


indolyl-3-acetic acid


indolyl-3-butyric acid


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Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • Thomas Gaspar
    • 1
  • Claire Kevers
    • 1
  • Hélène Bouillenne
    • 1
  • Yves Maziere
    • 2
  • Jean-Pierre Barbe
    • 2
  1. 1.Hormonologie fondamentale et appliquée, Institut de Botanique B 22Université de Liège — Sart TilmanLiègeBelgium
  2. 2.CommentryFrance

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