In vitro DNA footprinting

  • Pamela J. Green
  • Steve A. Kay
  • Eric Lam
  • Nam-Hai Chua
Chapter

Abstract

The analysis of DNA-protein interactions is an essential step towards elucidating molecular mechanisms for transcriptional regulation (for reviews, see [1–3]). Once a specific DNA sequence which serves as a binding site for a nuclear protein has been identified it can: (l)be correlated with the DNA sequences which mediate gene expression in vivo [4–6], (2) facilitate the construction of mutant and chimeric genes for further study [7], (3) be synthesized chemically and used to purify the factor by affinity chromatography [8], or (4) be used as a probe to identify cDNA clones of the binding protein from an expression library [9]. Factor isolation and clear functional correlations are prerequisites for unraveling the complex factor-factor interactions that ultimately control transcription.

Keywords

Total Cell Extract Klenow Enzyme Nuclear Extract Buffer Hexylene Glycol Nuclear Protein Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Kluwer Academic Publishers, Dordrecht 1989

Authors and Affiliations

  • Pamela J. Green
    • 1
  • Steve A. Kay
    • 1
  • Eric Lam
    • 1
  • Nam-Hai Chua
    • 1
  1. 1.Laboratory of Plant Molecular BiologyThe Rockefeller UniversityNew YorkUSA

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