Heterologous Flocculation of Escherichia Coli and Yeast
Mixed cell suspensions of Escherichia coli and Saccharomyces sp. spontaneously flocculated to an extent dependent upon environmental ionic strength. When separately suspended, the bacteria or yeast maintained theirmono dispersity over the range of ionic strengths in vestigated Maximal expression offlocculation was observed at an ionic strength of 0.0 15 sodium chloride and above.At ionic strengths of zero and 0.0015, mixed cell populations did not flocculate.Eventual floesize distribution swere a function of the initial concentrations of each species.Flocculation persisted irrespective of the presence of nutrient broth growth medium.
KeywordsIonic Strength Floc Size Cell Size Distribution Mixed Suspension Floc Formation
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- 1..Wang, D.I.C., Separation for Biotechology. In Separations for Biotechnology, ed. M.S. Verall and M.J. Hudson, Ellis Horwood, 1987, pp30–48.Google Scholar
- 3..Verway, E.J.W., and Overbeek, J.Th.G, Theory of stability of lyophobic colloids. Elsevier, Amsterdam, 1948.Google Scholar
- 4..Shaw, D.J., in Introduction to Colloid and Surface Chemistry. Butter worths, London, 1980.Google Scholar
- 5..Visser, J., Adhesion of colloidal particles. Surface and Colloid Science, 1976, 8, pp3–84.Google Scholar
- 6.James., The electrical properties and Topochemistry of Bacterial Cells. Adv Colloid Interface Science, 1982, 15, pp171–221.Google Scholar
- 8.Vincent, B., Young, C.A. and Tadros, Th, F.T., Equilibrium aspects of heterologous flocculation in mixed sterically stabilised dispersions. Faraday Discussions of the Chemical Society,Google Scholar