Separations for Biotechnology 2 pp 381-389 | Cite as
Performance of Anion-Exchange Cellulose at Process-Scale
Abstract
Whatman DE52 (tertiary amine substituted anion-exchange cellulose) has been shown to exhibit fast kinetics of adsorption and high capacity for proteins of molecular mass up to 670 kDa. These features lend themselves to process-scale use and we have investigated the operational parameters of process-scale chromatography of egg-white proteins in batch and column modes using DE52. We report that in a single pass adsorption step the efficiency of feedstock binding was 70% for batch adsorption compared with 78% for column adsorption. Desorption is more efficient in the column mode.
The binding capacity of DE52 was unaffected after 5 successive process-scale loadings although chromatographic performance was slightly reduced. This was restored using a clean-in-place procedure which simultaneously effects sterilisation and depyrogenation of the column and media.
Keywords
Soybean Trypsin Inhibitor Binding Efficiency Column Operation Chromatographic Performance Column ModePreview
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