Separations for Biotechnology 2 pp 142-151 | Cite as
Clarification of Tissue Culture Fluid and Cell Lysates Using Biocryl® Bioprocessing Aids
Abstract
Bioprocessing aids are used as direct additives during clarification (centrifugation or filtration) prior to chromatography. They improve the flocculation process, remove pyrogens, color bodies and acidic impurities (nucleic acids and acidic proteins) that can foul chromatography columns. This paper describes three examples of the uses of Biocryl bioprocessing aids: (1) a study of the clarification of pseudomonas cell lysate using bioprocessing aids as an alternative to heat treatment following high speed centrifugation; (2) a study of the effect of pre-chromatography polishing on peptide purification from a recombinant strain of yeast; and (3) DNA removal in the purification of monoclonal antibodies produced in tissue culture.
Keywords
High Performance Liquid Chromatography Color Body High Speed Centrifugation High Performance Liquid Chromatography Chromatogram Peptide UnitPreview
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