Improvements in Macroreticular Particles for Ion-Exchange and Gel-Permeation Chromatography
The effectiveness of chromatographic processes is often limited by the resistance to mass transfer into the exchanger particles. The likelihood of the exchange process being limited by particle diffusion resistance is especially high when one or both of the exchanging ions has a high molecular weight, and thus large molecular size and low mobility within the particles. Separation of ionic species from bioreactors is likely to involve such high molecular weight species and slow diffusion rates. Macroreticular particles are more effective in these applications because the larger species can diffuse more freely into the inner regions of the particles through large pores. The effects of small viscous flow rates through the larger pores of the macroreticular particle structure are examined in this paper to show how such flow can increase exchange rates. The importance of such flow is evaluated in terms of an effective diffusivity within the particle. The performance of an ion-exchange material can be affected strongly by the dimensions of its internal structure. The approach used to describe and evaluate the performance of ion-exchange materials with high-molecular-weight ions can be applied to macroreticular particles for separation of biological species and polymers by gel permeation.
KeywordsVoid Fraction Viscous Flow Effective Diffusion Coefficient Resin Particle Rapid Kinetic
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