Cryopreservation of Plant Cell Cultures

The Importance of Pretreatments
  • U. Seitz
  • D. Banspach
  • E. Göldner
  • E. Reinhard
Part of the Current Plant Science and Biotechnology in Agriculture book series (PSBA, volume 8)

Abstract

Cryopreservation experiments were performed with cell suspension cultures of the following species: Atriplex hortensis L.,.A, litoralis L. (Chenopodiaceae), Berberis wilsoniae HEMSL. & WILS. (Berberidaceae), Coleus blumei BENTH. (Lamiaceae), Daucus carota L. (Apiaceae), Digitalis lanata EHRH. (Scrophulariaceae), and Panax ginseng C.A. MEY. (Araliaceae), The protocol comprises a preculture phase in nutrient media with enhanced osmolarity, treatment with cryoprotectants, slow freezing, storage at -196°C, rapid thawing, and post-thaw treatments. Biochemical capacities remain unchanged in frozen-thawed cultures. This was shown to be the case in D. carota, D. lanata, and P. ginseng. A survey on literature concerning this point is presented. Some alternatives to the standard cryopreservation procedure are given. Experiments are underway to investigate the physiological events during the preculture phase. As a consequence of osmotic stress cell viability decreased to a certain extent. A suspension of this process and even recovery have been observed in the cases of positive response. Further events such as the uptake of preculture additives and changes in the cytoplasm: vacuole volume ratio will be discussed.

Keywords

Freeze Tolerance Rosmarinic Acid Panax Ginseng Plant Cell Culture Fluorescein Diacetate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • U. Seitz
    • 1
  • D. Banspach
    • 1
  • E. Göldner
    • 2
  • E. Reinhard
    • 1
  1. 1.Pharmazeutisches InstitutUniversität TübingenTübingenGermany
  2. 2.Boehringer MannheimPenzbergGermany

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