Light Activation of Membrane-Bound Fructose-1.6-Bisphosphatase from Pea Leaf Chloroplasts
Chloroplast fructose-1.6-bisphosphatase (FBPase) is one of the 13 enzymes of the Benson-Calvin cycle, all of them so far considered as solubles in the stromal space. However, the existence of some interactions with the thylakoid membranes has been reported in the last years concerning ribulose-1.5-bisphosphate carboxylase (1), FBPase (2,3), P-ribulokinase (4), and some other. In this context Kow and Gibbs (5) have obtained under mild conditions particulate preparations from spinach chloroplasts with low, but conclusive, CO2 assimilation capability without addition of any soluble stromal enzyme. On the other hand, loose association between some enzymes of the Benson-Calvin cycle has been also described (6).
KeywordsThylakoid Membrane Bisphosphate Carboxylase Intact Chloroplast Loose Association FBPase Activity
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