Operation of UQH2: CYT C2 Oxidoreductase of R. sphaeroides in the Steady State
Measurement of the thermodynamic poise of redox components of electron transfer chains under coupled conditions has been used extensively in the mitochondrial field to analyze through “cross-over points” the contributions to different spans to the work required to drive ATP synthesis, or maintain the proton gradient. In the work described in this paper, a steady state approach has been developed by which the redox poise of the electron transfer chain, the electron flux and H+-gradient could be simultaneously monitored, and the approach to the coupled steady-state (static head) could be followed kinetically on illumination of chromatophores from Rhodobacter sphaeroides.
KeywordsElectron Flux Proton Gradient Rhodobacter Sphaeroides Redox Center Electron Transfer Chain
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- 5.Cotton, N.P.J., Clark, A.J. and Jackson, J.B. (Eur. J. Biochem. 142, 193–198Google Scholar