Characterisation of a Full-Length cDNA Clone Encoding the Pea Rieske Fe-S Protein : Import and Processing by Isolated Chloroplasts

  • A. Hugh Salter
  • John C. Gray

Abstract

The cytochrome b-f complex of the chloroplast thylakoid membrane operates as a plastoquinol-plastocyanin oxidoreductase and is thus analogous to the mitochondrial cytochrome bc 1 complex with which it bears a large amount of structural and functional similarity. It consists in all higher plants of four components, which are cytochrome f, cytochrome b-563 the Rieske Fe-S protein, and a 17kDa protein (subunit IV)1. The cytochrome b-f complex has also been reported to contain small polypeptides1 and a chloroplast-encoded 5kDa polypeptide has recently been identified in maize and spinach2.The 20 kDa Rieske Fe-S protein may be removed from the isolated complex by hydroxyapatite chromatography in the presence of Triton X-1003.

Keywords

Derive Amino Acid Sequence 26kDa Precursor Rieske Protein Isolate Chloroplast eDNA Clone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Hurt, E. & Hauska, G.(1982) J.Bioenerg. Biomembr. 14, 405–424PubMedCrossRefGoogle Scholar
  2. 2.
    Haley, J. & Bogorad, L. (1989) Proc. Natl. Acad. Sci. USA 86, 1534–1538PubMedCrossRefGoogle Scholar
  3. 3.
    Hurt, E. et al (1981) FEBS Lett. 134, 1–5PubMedCrossRefGoogle Scholar
  4. 4.
    Willey, D.L. & Gray J.C. (1988) Photosyn. Res. 17, 125–144CrossRefGoogle Scholar
  5. 5.
    Alt, J. et al (1983) EMBO J. 2, 979–986PubMedGoogle Scholar
  6. 6.
    Tittgen, J. et al (1986) Mol. Gen. Genet. 204, 258–265CrossRefGoogle Scholar
  7. 7.
    Stepphun, J. et al (1987) Mol. Gen. Genet. 210, 171–177CrossRefGoogle Scholar
  8. 8.
    Salter, A.H. et al (1989) in Techniques and New Developments in Photosynthesis, (Malkin, R. & Barber, J. eds) pp. 473–476, Plenum, New YorkCrossRefGoogle Scholar
  9. 9.
    Pfefferkorn, B. and Meyer, H.E. (1987) FEBS Lett. 206, 233–237CrossRefGoogle Scholar
  10. 10.
    Phillips, A.L. and Gray, J.C. (1983) Eur. J. Biochem. 137, 553–560PubMedCrossRefGoogle Scholar
  11. 11.
    Newman, B.N. and Gray, J.C. (1988) Plant Mol. Biol. 10, 511–520CrossRefGoogle Scholar
  12. 12.
    Bartlett, S.G. et al (1982) in Methods in Chloroplast Molecular Biology (Edelman, M., Hallick R. B. & Chua N.-H., eds) pp. 1081–1092 Elsevier, Amsterdam.Google Scholar
  13. 13.
    Karlin-Neumann, G.A. & Tobin, E. (1986) EMBO J. 5, 9–13PubMedGoogle Scholar
  14. 14.
    Harnisch, U. et al (1985) Eur. J. Biochem. 149, 95–99PubMedCrossRefGoogle Scholar
  15. 15.
    Cline, J.F. et al (1985) J. Biol. Chem. 260, 3251–3254PubMedGoogle Scholar
  16. 16.
    Schagger, H. et al (1987) FEBS. Lett. 219, 161–168PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • A. Hugh Salter
    • 1
  • John C. Gray
    • 1
  1. 1.Botany SchoolUniversity of CambridgeCambridgeUK

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