Determination of the Intracellular Concentration of Inorganic Pyrophosphate in Rhodospirillum rubrum

  • Gaza F. Salih
  • Pål Nyrén

Abstract

Rhodospirillum rubrum is a purple, non-sulphur phototrophic bacterium. This organism is capable of catalyzing the synthesis of inorganic pyrophosphate (PPi) upon illumination. The phosphorylation of Pi to PPi is catalyzed by the membrane bound proton translocating inorganic pyrophosphatase (the H+-PPase) (1). PPi is also formed during the biosynthesis of cellular constituents such as proteins and nucleic acids. Beside the H+-PPase there is also a soluble PPase in R. rubrum (soluble PPases are found in all types of cells). The soluble enzyme only catalyze the hydrolysis of PPi not the synthesis, When PPi is hydrolyzed by the soluble PPase, the energy liberated upon hydrolysis of the anhydride bond is lost.

Keywords

Intracellular Concentration Soluble Enzyme Proton Motive Force Cellular Constituent Phototrophic Bacterium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Nyrén, P. (1985) Doctoral thesis, Univ. of Stockholm, Stockholm, Sweden.Google Scholar
  2. 2.
    Shakov, Yu.A., Nyrén, P. and Baltscheffsky, M. (1982) FEBS Lett. 146, 177–180.CrossRefGoogle Scholar
  3. 3.
    Nyrén, P. and Lundin, A. (1985) Anal. Biochem. 151, 504–509.PubMedCrossRefGoogle Scholar
  4. 4.
    Schuldinger, S., Padan, E., Rottenberg, H., Gromet-Elhanan, Z. and Avron, M. (1974) FEBS Lett. 49, 174–177.CrossRefGoogle Scholar
  5. 5.
    Moyle, J., Mitchell, R. and Mitchell, P. (1972) FEBS Lett. 23, 233.PubMedCrossRefGoogle Scholar
  6. 6.
    Flodgaard, H. and Fleron, P. (1974) J. Biol. Chem. 249, 3465–3470.PubMedGoogle Scholar
  7. 7.
    Leiser, M. and Gromet-Elhanan, Z. (1977) Arch. Biochem. Biophys. 178, 79–88.PubMedCrossRefGoogle Scholar
  8. 8.
    Bashford, C.L., Baltscheffsky, M. and Prince, R.C. (1979) FEBS Lett. 97, 55–59.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Gaza F. Salih
    • 1
  • Pål Nyrén
    • 1
  1. 1.Department of Biochemistry, Arrhenius LaboratoriesUniversity of StockholmStockholmSweden

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