pH-Dependent Changes in ATP and ADP Affinity for the Tight Nucleotide-Binding Site of the Chloroplast Coupling Factor CF1

  • A. N. Malyan
  • O. I. Vitseva

Abstract

The ATPase complex of chloroplasts (H+-ATPase, ATP-synthase) carries out synthesis (hydrolysis) of ATP coupled with transmembrane transport of hydrogen ions. This complex consists of a hydrophytic catalytic part called coupling factor CF1, and a hydrophobic part, CF0, the function of which is to translocate protons towards CF1. CF1 can bind as many as six nucleotide molecules /1/. After CF1 precipitation by ammonium sulfate with subsequent gel filtration, the enzyme retains about 1 mol of tightly bound nucleotides consisting mainly of ADP /2/.

Keywords

Hydrophobic Part Transmembrane Transport ATPase Complex Free Nucleotide Chloroplast Coupling Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • A. N. Malyan
    • 1
  • O. I. Vitseva
    • 1
  1. 1.Inst. of Soil Science and PhotosynthesisAcad. Sci. USSRPushchino, Moscow RegionUSSR

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