The Thermodynamic Characteristic of Four-Heme Cytochrome C from Rhodopseudomonas Viridis Reaction Centers

  • S. M. Dracheva
  • A. L. Drachev
  • V. P. Shinkarev

Abstract

Optical studies on Rps. viridis RCs provided evidence that all four hemes of cytochrome c have different absorption spectra in the α-band domain as well as midpoint potentials [1–4]. In this work we used the individual spectra of each heme and linear least-squares method to elucidate the quantity of every heme from full absorption spectra of cytochrome c in the α-band region as function of redox potential. For all four hemes the values of midpoint potentials established by the method of decomposition are in good agreement with those determined earlier [1].

Keywords

Redox Potential Component Approximation Differential Spectrum Midpoint Potential Full Absorption 
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References

  1. 1.
    Dracheva, S.M., Drachev, L.A., Konstantinov, A.A., Semenov, A.Yu., Skulachev, V.P., Arutjunjan, A.M., Shuvalov, V.A. and Zaberezhnaya, S.M.(1988) Eur. J. Biochem. 171, 253–264PubMedCrossRefGoogle Scholar
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    Alegria G. and Dutton, P.L. (1987) in Cytochrome Systems (Papa, S., Chance, B. and Ernster, L., Eds.) pp 601–608, Plenum Press, New York and LondonCrossRefGoogle Scholar
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    Vermeglio, A., Richaud, P. and Breton, J. (1989) FEBS Lett. 243, 259–263CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • S. M. Dracheva
    • 1
    • 2
  • A. L. Drachev
    • 1
    • 2
  • V. P. Shinkarev
    • 1
    • 2
  1. 1.Institute of MicrobiologyRussia
  2. 2.Moscow State UniversityRussia

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