Pulsed EPR Studies of the Manganese Center of the Oxygen-Evolving Complex of Photosystem II

  • R. David Britt
  • Victoria J. Derose
  • Vittal K. Yachandra
  • Dennis H. Kim
  • Kenneth Sauer
  • Melvin P. Klein

Abstract

The pulsed EPR technique of Electron Spin Echo Envelope Modulation (ESEEM) is used to measure the nuclear transition frequencies of paramagnetic nuclei magnetically coupled to unpaired electron spins.1 We have employed this technique to study the Mn center of the Photosystem II oxygen-evolving complex. ESEEM measurements were performed on the “multiline” Mn EPR signal associated with the S 2 state of the Kok cycle.

Keywords

Electron Spin Echo Envelope Modulation PSII Membrane PSII Preparation Electron Spin Echo Envelope Modulation Spectrum Electron Spin Echo Envelope Modulation Experiment 
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References

  1. 1.
    Mims, W. B. and Peisach, J. (1981) in Biological Magnetic Resonance (Berliner, L. J. and Reuben, J., eds.), Vol. 3, pp. 213–263, Plenum, New YorkGoogle Scholar
  2. 2.
    Britt, R. D. (1988) Ph.D. Thesis, Department of Physics, University of California at BerkeleyGoogle Scholar
  3. 3.
    Britt, R. D., Zimmermann, J. L., Sauer, K., and Klein, M. P. (1989) J. Am. Chem. Soc. 111, 3522–3532CrossRefGoogle Scholar
  4. 4.
    McDermott, A. E., Yachandra, V. K., Guiles, R. D., Cole, J. L., Dexheimer, S. L., Britt, R. D., Sauer, K., and Klein, M. P. (1988) Biochemistry 27, 4021–4031PubMedCrossRefGoogle Scholar
  5. 5.
    Kevan, L. (1979) in Time Domain Electron Spin Resonance (Kevan, L. and Schwartz, R. N., eds.), pp. 277–341, Wiley, New YorkGoogle Scholar
  6. 6.
    Serpersu, E. H., McCracken, J., Peisach, J., and Mildvan, A. S. (1988) Biochemistry 27, 8034–8044PubMedCrossRefGoogle Scholar
  7. 7.
    Hansson, Ö., Andréasson, L.-E., and Vänngård, T. (1986) FEBS Lett. 195, 151–154CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • R. David Britt
    • 1
  • Victoria J. Derose
    • 1
  • Vittal K. Yachandra
    • 1
  • Dennis H. Kim
    • 1
  • Kenneth Sauer
    • 1
  • Melvin P. Klein
    • 1
  1. 1.Laboratory of Chemical BiodynamicsLawrence Berkeley LaboratoryBerkeleyUSA

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