Gelelectrophoretic Gliadin Patterns of Euplasmic and Alloplasmic Primary Triticale and the Corresponding Wheat Parents

  • Thomas Günther
  • Claus-Ulrich Hesemann
  • Gitta Oettler
Part of the Developments in Plant Breeding book series (DIPB, volume 5)

Summary

This study was conducted to determine the influence of wheat cytoplasms on storage protein patterns in wheat and triticale. Euplasmic and alloplasmic triticale had been produced by crossing T. durum and T. aestivum, having nine different cytoplasms, with four rye inbred lines. The gliadin patterns of these euplasmic and alloplasmic primary hexa- and octoploid triticale and the corresponding wheat parents were biochemically analysed, using the acid PAGE as electrophoretic technique. Comparing the gliadin patterns of the euplasmic and alloplasmic wheat parents it was found that the variation of the quantitative expression in the gliadin patterns was amplified in the background of alien wheat cytoplasms. This variation was reduced in alloplasmic triticale, especially in hexaploids. The quantitative expression of rye secalin bands in triticale was influenced by the wheat genome. From this extensive study, it can be concluded that alien wheat cytoplasms produce quantitative and qualitative variations in the expression of gliadin patterns.

Keywords

Single Kernel Common Wheat Cultivar Wheat Parent Durum Line Gliadin Allele 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Sage GCM. Nucleo-cytoplasmic relationships in wheat. Adv Agron 1976; 28: 267–300.CrossRefGoogle Scholar
  2. 2.
    Mathias RJ, Fukui K, Law CN. Cytoplasmic effects on the tissue culture response of wheat (Triticum aestivum) callus. Theor Appl Genet 1986; 72: 70–75.CrossRefGoogle Scholar
  3. 3.
    Bushuk W, Zillman RR. Wheat cultivar identification by gliadin electrophoregrams. I. Apparatus, method and nomenclature. Can J Plant Sci 1978; 58: 505–515.CrossRefGoogle Scholar
  4. 4.
    Du Cros DL Wrigley CW. Improved electrophoretic methods for identifying cereal varieties. J Sci Food Agric 1979; 30: 785–794.CrossRefGoogle Scholar
  5. 5.
    Dal Belin Peruffo A, Pallavicini C, Varanini Z, Pogna, NE. Analysis of wheat varieties by gliadin electrophoregrams I.Catalogue of electrophoregram formulas of 29 common wheat cultivars grown in Italy. Genet Agr 1981; 35: 195–208.Google Scholar
  6. 6.
    Metakovsky EV, Kudryavtsev AM, Iakobashvili ZA, Novoselskaya AYu. Analysis of phylogenetic relations of durum, carthlicum and common wheats by means of alleles of gliadin coding loci. Theor Appl Genet 1989; 77: 881–887.CrossRefGoogle Scholar
  7. 7.
    Van de Weghe L. Comparative study of electrophoretic methods for cultivar identification of wheat and triticale. Seed Sci & Technol 1991; 19: 41–50.Google Scholar
  8. 8.
    Skeritt JH, Martimizzi O, Metakovsky EV. Chromosomal control of wheat gliadin protein epitopes: analysis with specific monoclonal antibodies. Theor Appl Genet 1991; 82: 44–53.CrossRefGoogle Scholar
  9. 9.
    Federmann G, Goecke EU, Steiner AM. Der elektrophoretische Nachweis von Weichweizen (Triticum aestivum L.) in Dinkelmehlen (Triticum spelta L.). Getreide Mehl und Brot 1992; 46 (10): 309–312.Google Scholar
  10. 10.
    Metakovsky EV. Gliadin allele identification in common wheat II. Catalogue of gliadin alleles in common wheat. J Genet & Breed 1991; 45: 325–344.Google Scholar

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Thomas Günther
    • 1
  • Claus-Ulrich Hesemann
    • 2
  • Gitta Oettler
    • 3
  1. 1.Research Centre Biotechnology and Plant BreedingUniversity of HohenheimGermany
  2. 2.Institute of GeneticsUniversity of HohenheimGermany
  3. 3.State Plant Breeding InstituteUniversity of HohenheimGermany

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