Regeneration and transformation of sugarbeet by Agrobacterium tumefaciens
Sugarbeet, Beta vulgaris L., is of significant commercial importance as the major sucrose producer in temperate climates and world-wide currently contributes about 35% of the total sucrose yield . Sugarcane, a monocot, is limited to warmer climates, and so the two species can be considered complementary in their global distribution. Sugarbeet has also attracted further attention, by virtue of the potential to accumulate novel or valuable specific metabolites in the storage tissues: in other words, to use beets, as ‘green bioreactors’ for the synthesis of products other than sucrose. Sugarbeet is a natural cross-pollinator, and so modern cultivars are highly heterozygous. It is also a biennial species, and both these features contribute to a slow rate of progress in the generation of new varieties by conventional breeding. A genetic engineering strategy for sugarbeet therefore would be expected to aid the breeder in introducing specific traits directly into ‘elite’ (commercially valuable) genotypes and also would open up the possibility of using sugarbeet to produce metabolites novel to that species.
KeywordsShoot Culture Calcium Hypochlorite Genetic Engineering Strategy Nurse Culture Transgenic Sugar Beet
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