Nuclear DNA Primers

  • Joanne Russell
  • Gordon C. Machray
  • Robbie Waugh
Chapter

Abstract

Since its introduction in 1985, the polymerase chain reaction (PCR) (1) has profoundly affected the way in which molecular studies are conducted. Olsen et al. (2) first proposed that short single copy DNA sequences could be amplified by PCR from cloned DNA using specific primers. This requires sequencing only a short tract of DNA from any cloned sequence. Primers for PCR can therefore be constructed by sequencing anonymous cDNA or genomic clones, clones used in mapping projects, RAPD fragments or previously published DNA sequences. Here we will consider only the latter source, as ‘probe isolation’ is covered in Chapters 12.2 and 12.3 and DNA sequencing in Chapter 8.2.

Keywords

Sickle Cell Anemia Output File European Bioinformatics Institute Sequence Retrieval Barley Chloroplast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Saiki, R.K., Scharf, S.J., Faloona, F., Mullis, K.B., Horn, G.T., Erlich, H.A. and Arnheim, N. (1985) Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science, 230, 1350–1354.CrossRefGoogle Scholar
  2. 2.
    Olsen, M., Hood, L., Cantor, C. and Botsein, D. (1989) A common language for physical mapping of the human genome. Science, 245, 1434–1435.CrossRefGoogle Scholar
  3. 3.
    Devereaux, J., Haeberli, P. and Smithies, O. (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research, 12, 387–395.CrossRefGoogle Scholar
  4. 4.
    Rychlik, W. and Rhoads, E. (1989) A computer program for choosing optimal oligonucleotides for filter hybridization, sequencing and in vitro amplification. Nucleic Acids Research, 17, 8543–8551.CrossRefGoogle Scholar

Copyright information

© Chapman & Hall 1998

Authors and Affiliations

  • Joanne Russell
  • Gordon C. Machray
  • Robbie Waugh

There are no affiliations available

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