Genotyping with Microsatellite Markers

  • Claudio Ciofi
  • Stephan M. Funk
  • Trevor Coote
  • David J. Cheesman
  • Robert L. Hammond
  • Ilik J. Saccheri
  • Michael W. Bruford
Chapter

Abstract

Microsatellites — also known as short tandem repeats (STRs) or simple sequence repeats (SSRs) — consist of tandemly repeated units, each between one and 10 base-pairs in length, such as (TG)n or (AAT)n (1). They are widely dispersed throughout eukaryotic genomes and are often highly polymorphic due to variation in the number of repeat units. The high information content of the genetic data yielded by microsatellite loci, and the advantage that material for microsatellite analysis can potentially be sampled non-invasively from free-living populations (2) make these markers one of the molecular tools of choice for many population and biodiversity studies. A protocol for the isolation of microsatellites is given in Chapter 15.1 and the characteristics of microsatellite loci in eukaryotic genomes are summarized in Box 11.1.

Keywords

Microsatellite Locus Power Pack Geiger Counter Allele Size Range Native Allele 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman & Hall 1998

Authors and Affiliations

  • Claudio Ciofi
  • Stephan M. Funk
  • Trevor Coote
  • David J. Cheesman
  • Robert L. Hammond
  • Ilik J. Saccheri
  • Michael W. Bruford

There are no affiliations available

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