Genotyping with Microsatellite Markers
Microsatellites — also known as short tandem repeats (STRs) or simple sequence repeats (SSRs) — consist of tandemly repeated units, each between one and 10 base-pairs in length, such as (TG)n or (AAT)n (1). They are widely dispersed throughout eukaryotic genomes and are often highly polymorphic due to variation in the number of repeat units. The high information content of the genetic data yielded by microsatellite loci, and the advantage that material for microsatellite analysis can potentially be sampled non-invasively from free-living populations (2) make these markers one of the molecular tools of choice for many population and biodiversity studies. A protocol for the isolation of microsatellites is given in Chapter 15.1 and the characteristics of microsatellite loci in eukaryotic genomes are summarized in Box 11.1.
KeywordsMicrosatellite Locus Power Pack Geiger Counter Allele Size Range Native Allele
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- 2.Morin, PA & DS Woodruff (1996). Noninvasive genotyping for vertebrate conservation. Molecular genetic approaches in conservation, RK Wayne & TB Smith, eds., Oxford University Press, New York, pp. 298–313.Google Scholar
- 10.Slatkin, M (1995). A measure of population subdivision based on microsatellite allele frequency. Genetics 139, 457–462.Google Scholar
- 11.Goldstein, DB, AR Linares, LL Cavalli-Sforza & MW Feldman (1995). An evaluation of genetic distances for use with microsatellite loci. Genetics 139, 463–471.Google Scholar
- 12.Coote, T & MW Bruford (1997). Universally applicable microsatellites for analysis of genetic variation in apes and Old World monkeys. Journal of Heredity. 87, 406–410.Google Scholar