When first published in 1975, RFLP procedures used probes labelled with radioisotopes to detect complementary sequences in complex mixtures of restriction fragments (1). During the early 1980s, several groups experimented with nonradioactive probing systems (2, 3). Since then, several commercially available kits have been released that are at least as sensitive as radioisotopes, and which yield results in much shorter times. It is beyond the scope of this section to provide the reader with detailed protocols for the use of nonradioactive probes, but the subject has been reviewed recently (4) and general textbooks are available (5).
KeywordsComplementary Sequence Detailed Protocol Antibody Conjugate Charge Nylon Membrane Careful Handling
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