Proteomics Advances in the Study of Leishmania Parasites and Leishmaniasis

  • Jose Batista de Jesus
  • Camila Mesquita-Rodrigues
  • Patricia Cuervo
Chapter
Part of the Subcellular Biochemistry book series (SCBI, volume 74)

Abstract

Leishmania spp. are digenetic parasites which cause a broad spectrum of fatal diseases in humans. These parasites, as well as the other trypanosomatid, regulate gene expression at the post-transcriptional and post-translational levels, so that a poor correlation is observed between mRNA content and translated proteins. The completion of the genomic sequencing of several Leishmania species has enormous relevance to the study of the leishmaniasis pathogenesis. The combination of the available genomic resources of these parasites with powerful high-throughput proteomic analysis has shed light on various aspects of Leishmania biology as well as on the mechanisms underlying the disease. Diverse proteomic approaches have been used to describe and catalogue global protein profiles of Leishmania spp., reveal changes in protein expression during development, determine the subcellular localization of gene products, evaluate host-parasite interactions and elucidate drug resistance mechanisms. The characterization of these proteins has advanced, although many fundamental questions remain unanswered. Here, we present a historic review summarizing the different proteomic technologies applied to the study of Leishmania parasites during the last decades and we discuss the proteomic discoveries that have contributed to the understanding of Leishmania parasites biology and leishmaniasis.

Keywords

Protein Spot Visceral Leishmaniasis Cutaneous Leishmaniasis Resistant Parasite Leishmania Species 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

1DE

One dimensional electrophoresis

2D-DIGE

Two dimensional differential gel electrophoresis

2DE

Two dimensional electrophoresis

2D-LC-MS/MS

Two dimensional liquid chromatography coupled with tandem mass spectrometry

ATP

Adenosine triphosphate

BLAST

Basic local alignment search tool

DNA

Deoxyribonucleic acid

EDTA

Ethylenediamine tetra acetic acid

ESI

Electrospray ionization

ESI-MS

Electrospray ionization mass spectrometry

GFP

Green fluorescent protein

GO

Gene ontology

GP46

Glycoprotein 46

GP63

Glycoprotein 63 also called leishmanolysin and major surface peptidase

GTP

Guanosine triphosphate

HSP60

Heat shock protein 60

HSP70

Heat shock protein 70

HSP90

Heat shock protein 90

HSP100

Heat shock protein 100

IEF

Isoelectric focalization

IgG

Immunoglobulin g

IgM

Immunoglobulin m

iMAC

Metal affinity chromatography

iTRAQ

Isobaric tags for relative and absolute quantification

KMP-11

Kinetoplastid membrane protein 11

LC-MS/MS

Liquid chromatography coupled with tandem mass spectrometers

MALDI

Matrix assisted laser desorption ionization

MALDI-MS

Matrix assisted laser desorption ionization mass spectrometry

MALDI-MS/MS

Matrix assisted laser desorption ionization tandem mass spectrometry

mRNA

Messenger ribonucleic acid

MUDPIT

Multidimensional protein identification

PTMs

Post translational modifications

Rab7

Ras-related protein 7

RNA

Ribonucleic acid

SbIII

Trivalent antimonials

SbV

Pentavalent antimonials

SDS-PAGE

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

SHERP

Small hydrophilic endoplasmic reticulum-associated protein

SILAC

Stable isotope labeling by amino acids in cell culture

SKRP

Small-kinetoplastid-related-protein

TCA

Trichloroacetic acid

Th1

T helper 1

WHO

World health organization

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Jose Batista de Jesus
    • 1
    • 2
  • Camila Mesquita-Rodrigues
    • 2
  • Patricia Cuervo
    • 3
  1. 1.Departamento de Engenharia de BiossistemasUniversidade Federal de São João Del ReiSão João Del ReiBrazil
  2. 2.Laboratório de Biologia Molecular e Doenças EndêmicasInstituto Oswaldo Cruz, FIOCRUZRio de JaneiroBrazil
  3. 3.Laboratório de Pesquisa em LeishmanioseInstituto Oswaldo Cruz, FIOCRUZRio de JaneiroBrazil

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