GP63 Function in the Interaction of Trypanosomatids with the Invertebrate Host: Facts and Prospects
The GP63 of the protozoan parasite Leishmania is a highly abundant zinc metallopeptidase, mainly glycosylphosphatidylinositol-anchored to the parasite surface, which contributes to a myriad of well-established functions for Leishmania in the interaction with the mammalian host. However, the role of GP63 in the Leishmania-insect vector interplay is still a matter of controversy. Data from GP63 homologues in insect and plant trypanosomatids strongly suggest a participation of GP63 in this interface, either through nutrient acquisition or through binding to the insect gut receptors. GP63 has also been described in the developmental forms of Trypanosoma cruzi, Trypanosoma brucei and Trypanosoma rangeli that deal with the vector. Here, the available data from GP63 will be analyzed from the perspective of the interaction of trypanosomatids with the invertebrate host.
KeywordsGP63 Gene Bloodstream Form Invertebrate Host Procyclic Form Metacyclic Trypomastigotes
Ethylene glycol tetraacetic acid
Human immunodeficiency virus
Major surface peptidase
Procyclic acidic repetitive protein
Promastigote surface peptidase
Glycosylphosphatidylinositol-anchored variant surface protein
This study was supported by grants from the following Brazilian Agencies: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (MCT/CNPq), Fundação de Amparo à Pesquisa no Estado do Rio de Janeiro (FAPERJ) and Fundação Oswaldo Cruz (FIOCRUZ).
- d’avila-Levy CM, Santos ALS, Cuervo P et al (2012) Applications of zymography (substrate-SDS-PAGE) for peptidase screening in a post-genomic era. In: Magdeldin S (Org) Gel electrophoresis – advanced techniques, 1ed. In Tech, RijekaGoogle Scholar
- Etges RJ, Bouvier J, Bordier C (1986) The major surface protein of Leishmania promastigotes is a protease. J Biol Chem 261:9099–9101Google Scholar
- McGwire BS, Chang KP (1996) Posttranslational regulation of a leishmania HEXXH metalloprotease (gp63). The effects of site specific mutagenesis of catalytic, zinc binding, N-glycosylation, and glycosyl phosphatidylinositol addition sites on N-terminal end cleavage, intracellular stability, and extracellular exit. J Biol Chem 271:7903–7909PubMedCrossRefGoogle Scholar
- Teixeira MM, Borghesan TC, Ferreira RC et al (2011) Phylogenetic validation of the genera Angomonas and Strigomonas of trypanosomatids harboring bacterial endosymbionts with the description of new species of trypanosomatids and of proteobacterialsymbionts. Protist 162:503–524PubMedCrossRefGoogle Scholar
- Wilson V, Southern B (1979) Lizard leishmania. In: Lumsden W, Evans D (eds) Biology of kinetoplastida. Academic, New YorkGoogle Scholar