The Legionella pneumophila Chaperonin 60 and the Art of Keeping Several Moonlighting Jobs
The title of this chapter intends to emphasize the fact that, as the essential chaperonin 60 of the bacterial pathogen Legionella pneumophila, HtpB has a main day job in protein folding, in addition to several alternate night jobs, depending on where it is located. The alternate virulence-related jobs that we have described for HtpB include the interaction with host cell surface receptors (which results in signaling and a variety of host cell responses), attraction of host cell mitochondria, modification of the host cell actin cytoskeleton, induction of bacterial filamentation, and interaction with specific host cell proteins, i.e. S-adenosyl methionine decarboxylase (which plays a role in the synthesis of host cell polyamines). These alternate HtpB jobs were primarily discovered as strong phenotypes after expression of recombinant HtpB in bacteria, yeast and mammalian cells. It is fascinating that HtpB possesses the extraordinary ability to functionally adapt to the disparate cellular environments of prokaryotic and eukaryotic cells. Although we have not yet been able to decipher the molecular basis for this adaptation, HtpB stands out as an artful moonlighting chaperonin capable of serving several night jobs.
KeywordsHost Cell Cytoplasmic Side Bordetella Pertussis Bacterial Cell Surface Host Cell Cytoplasm
- Allan DS (2002) Secretion of Hsp60 chaperonins (GroEL) homologs by Legionella pneumophila. MSc thesis, Dalhousie University, Halifax, NS, CanadaGoogle Scholar
- Chong A, Riveroll A, Allan DS, Garduño E, Garduño RA (2006) The Hsp60 chaperonin of Legionella pneumophila: an intriguing player in infection of host cells. In: Cianciotto NP, Abu Kwaik Y, Edelstein PH, Fields BS, Geary DF, Harrison TG, Joseph CA, Ratcliff RM, Stout JE, Swanson MS (eds) Legionella: state of the art 30 years after its recognition. ASM Press, Washington, DC, pp 255–260Google Scholar
- Nasrallah GK, Riveroll AL, Chong A, Murray LE, Lewis PJ, Garduño RA (2011b) Legionella pneumophila requires polyamines for optimal intracellular growth. J Bacteriol 193:4346–4360. Author correction for this paper: (2012) J Bacteriol 194:3032Google Scholar
- Riveroll AL (2005) The Legionella pneumophila chaperonin – an investigation of virulence-related roles in a yeast model. PhD thesis, Dalhousie University, Halifax, NS, CanadaGoogle Scholar