Histoplasma capsulatum Chaperonin 60: A Novel Adhesin and Vaccine Candidate

  • Joshua Daniel Nosanchuk
  • Allan Jefferson Guimarães
Chapter
Part of the Heat Shock Proteins book series (HESP, volume 7)

Abstract

HSP60 has a key role in immunoregulation and the abundance of HSP60 proteins in mammalian and microbial cells impacts diverse biological functions in both. Therefore, it may be essential for innate immune cells to distinguish HSP60 proteins by their endogenous or infectious origin. Histoplasma capsulatum (Hc) is able to express an Hsp60 on its surface that facilitates engagement with macrophages through complement 3 receptors (CR3) and leads to phagocytosis of the fungus where the pathogen survives within phagosomes. HcHsp60 plays important metabolic roles related to the fungal adaptation to temperature and oxidative stress conditions. Hc is also able to secrete this chaperone, which could play important roles in modulation of the antifungal immunological responses. Vaccination with recombinant HcHsp60 has conferred protection in mouse models against intravenous and pulmonary infections by Hc. Passive immunization using monoclonal antibodies to HcHsp60 has provided excellent results against Hc in mouse models, mainly by activating the antifungal functions of macrophages and inducing a Th-1 type immunoresponse. As Hsp60 is a common fungal antigen, passive immunization with Hsp60 mAbs has been evaluated against distinct fungal pathogens, including coupling the antibody with radionuclides (radioimmunotherapy) in order to increase the antifungal potential of these mAbs. In this chapter, we detail the research progresses on Hsp60 of Hc with particular focus on its characterization as an adhesin and utilization in vaccination and passive immunization.

Keywords

Invasive Fungal Infection Heat Shock Response Passive Immunization Histoplasma Capsulatum Yeast Phase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Joshua Daniel Nosanchuk
    • 1
  • Allan Jefferson Guimarães
    • 2
  1. 1.Department of Microbiology and Immunology and Department of MedicineAlbert Einstein College of Medicine of Yeshiva UniversityBronxUSA
  2. 2.Instituto Biomédico, Dept. de Microbiologia e Parasitologia - MIPUniversidade Federal FluminenseNiteróiBrazil

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