Radioprotective Properties of Selenomethionine with Methionine, Extracts from Basidium Fungi and Exogenous DNA

  • Alexander D. Naumov
  • Natalia I. Timokhina
  • Alexandra V. Litvinchuk
  • Gennadii G. Vereshchako
  • Alina M. Khodosovskaya
  • Svetlana N. Sushko
  • Elena M. Kadukova
Conference paper
Part of the NATO Science for Peace and Security Series A: Chemistry and Biology book series (NAPSA)

Abstract

The search for effective substances to provide protection to the organism under radiation treatment continues to be one of the most important directions for studies in radiobiology. The results of the study show that selenmethionine in the indicated doses with methionine (4 mg/kg) have pronounced efficiency on the defense of organisms against acute irradiation at a dose of 2 Gy. This was shown in the recovery of leucocytes in the blood, the increase in the total number of spermatogenic cells, and a significant increase in the spermatid count by 2 months after irradiation (2.3–2.6 times). This may provide a restoration in the process of spermatogenesis as well as normalization of lipid peroxidation processes and transamination enzymes activities in the irradiated animals. We also studied the anti-tumoral and radioprotective activities of the mushroom aqueous extracts. The antitumoral properties of Flammulina velutipes are shown. Its aqueous extracts decrease a spontaneous level of adenomas and, as well as Auricularia auricular-judae, reduce the yield of urethane-induced adenomas. The aqueous extracts of Flammulina velutipes and Phallus impudicus demonstrate radioprotective properties, i.e. reduce a number of adenomas in irradiated mice, raise mice survival and stimulate a growth of endogenous colonies in spleen. We prepared and studied the exogenous DNA. Micronuclei tests of bone marrow show an improvement in the DNA repair process in erythroblasts of irradiated and treated animals. Based on PCR analysis, it was suggested that the exogenous DNA substance can protect cardiac cells from the activation of pro-apoptotic gene Bax, and it can rescue the heart from irradiation-induced apoptosis. Thus the studied substances can be promising agents for protection and recovery of an organism from radiation injury.

Keywords

Aqueous Extract Spermatogenic Cell Irradiate Mouse Spleen Weight Radioprotective Effect 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Vereschako GG, Khodosovskaya A, Konoplya E (2011) Radiation protection of male reproductive system: state of the problem and questions arisen after the Chernobyl accident. Uspechi Sovr Biol (in Russian) 131:16–29Google Scholar
  2. 2.
    Baraboi VA (2004) Biological functions, metabolism and mechanisms of selenium effects. Uspechi Sovr Biol (in Russian) 124:157–168Google Scholar
  3. 3.
    Miretskij GI, Danitskaya E, Troitskay M et al (1984) Methionin – possible means of prophylaxis remote consequences of irradiation. Hyg Sanit (in Russian) 7:83–85Google Scholar
  4. 4.
    Mamina VP, Semenov D (1976) Method of determination of testes spermatogonic cell amount in cell suspension. Cytology (in Russian) 18:913–914Google Scholar
  5. 5.
    Evdokimov VV, Kodencova V et al (1997) Vitamin state and rats spermatogenese in late-term after irradiation of different doses. Bul Exper Biol Med (in Russian) 23:524–527Google Scholar
  6. 6.
    Stalnaya ID, Garishvili T (1977) Modern methods in biochemistry (in Russian). Medicine, MoscowGoogle Scholar
  7. 7.
    Shchelkunov LF, Dudkin M, Golubkina M et al (2000) Selen and its role in nutrition. Hyg Sanit (in Russian) 5:32–35Google Scholar
  8. 8.
    Wasser SP, Nevo E, Sokolov D et al (2000) Dietary supplements from medicinal mushrooms: diversity of types and variety of regulations. Int J Med Mushrooms 2:1–19CrossRefGoogle Scholar
  9. 9.
    Wasser SP (2002) Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl Microbiol Biotechnol 60(3):58–74Google Scholar
  10. 10.
    Monro JA (2003) Treatment of cancer with mushroom products. Arch Environ Health 58(8):533–537PubMedCrossRefGoogle Scholar
  11. 11.
    Kobayashi H (2005) Suppressing effects of daily oral supplementation of β-glucan extracted from Agaricus blazei Murill on spontaneous peritoneal disseminated metastasis in mouse model. J Cancer Res Clin Oncol 131(8):527–538PubMedCrossRefGoogle Scholar
  12. 12.
    Inoue A, Kodama N, Nanba H (2002) Soy isoflavone aglycone modulates a hematopoietic response in combination with soluble β-glucan: SCG. Biol Pharm Bull 25(4):536–540PubMedCrossRefGoogle Scholar
  13. 13.
    Ho JC, Konerding MA, Gaumann A et al (2004) Fungal polysaccharopeptide inhibits tumor angiogenesis and tumor growth in mice. Life Sci 75(11):1343–1356PubMedCrossRefGoogle Scholar
  14. 14.
    Voronin АY, Kulikov VY (2004) Colony-formation in spleens of experimental animals under exposure of geomagnetic field with very low strength. Bull СО RAMS (in Russian) 1(111):73–76Google Scholar
  15. 15.
    Bergsmedh A, Szeles A, Henricksson M et al (2001) Horizontal transfer of oncogenes by uptake of apoptotic bodies. Proc Natl Acad Sci USA 98(11):6407–6411PubMedCrossRefGoogle Scholar
  16. 16.
    Vasquez KM, Marburger K, Intody Z et al (2001) Manipulating the mammalian genome by homologous recombination. Proc Natl Acad Sci USA 98:8403–8410PubMedCrossRefGoogle Scholar
  17. 17.
    van Attikum H, Bundock P, Hooykaas PJJ (2001) Non-homologous end-joining proteins are required for Agrobacterium T-DNA integration. EMBO J 20(22):6550–6558PubMedCrossRefGoogle Scholar
  18. 18.
    Lin FL, Sperle KM, Sternberg NL (1990) Extrachromosal recombination in mammalian cells as studied with single and double-stranded DNA fragments during transfer of DNA into mouse L cells. Mol Cell Biol 10:113–119PubMedGoogle Scholar
  19. 19.
    Likhacheva AS, Nikolin VP, Popova NA et al (2007) Integration of human DNA fragments into the cell genomes of certain tissues from adult mice treated with cytostatic cyclophosphamide in combination with human DNA. Gene Ther Mol Biol 11:185–202Google Scholar
  20. 20.
    Likhacheva AS, Nikolin VP, Popova NA et al (2007) Exogenous DNA can be captured by stem cells and be involved in their rescue from death after lethal-dose γ-radiation. Gene Ther Mol Biol 11:305–314Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Alexander D. Naumov
    • 1
  • Natalia I. Timokhina
    • 1
  • Alexandra V. Litvinchuk
    • 1
  • Gennadii G. Vereshchako
    • 1
  • Alina M. Khodosovskaya
    • 1
  • Svetlana N. Sushko
    • 1
  • Elena M. Kadukova
    • 1
  1. 1.Institute of Radiobiology of the National Academy of Sciences of BelarusGomelBelarus

Personalised recommendations