Regenerative Therapies-Trachea

Chapter

Abstract

No preferred treatment, which could offer a functional solution, has been so far developed for patients affected by extensive airway damages. As the field of tissue engineering attempts to develop tracheal replacements, multiple types and combinations of cells, scaffold materials, and/or culture conditions have been used. Interest has been evoked by decellularized natural matrices, which affecting cell proliferation, migration and differentiation, could play an active part in tissue regeneration and remodeling. Using the detergent-enzymatic method, we were able to obtain decellularized human tracheal matrices lacking MHC antigens (bypassing rejection), having structural, mechanical and in vivo pro-angiogenic properties similar to that of native airways and supporting in vivo recellularization. Starting from these results, we have developed an in vivo tissue engineered strategy, based on airway bioengineered grafts combined with autologous stem cells and pharmacological intervention (to boost progenitor cell recruitment commitment), which resulted to be a clinically successful alternative for patients with serious airway disorders.

Keywords

Adenoid Cystic Carcinoma Tracheal Stenosis Congenital Tracheal Stenosis Cartilaginous Ring Tracheal Reconstruction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

DEM

detergent-enzymatic method

EPO

erythropoietin

G-CSF

granulocyte colony-stimulating factor

MSCs

marrow stromal cells

TGF-β

transforming growth factor-β

TNF-α

tumour necrosis factor-α

β-FGF

basic fibroblast growth factor

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.BIOAIRlab (Laboratory of Biomolecular and Bioengineering Airway), Department of General Thoracic and Regenerative Surgery and Intrathoracic BiotransplantationUniversity Hospital CareggiFlorenceItaly
  2. 2.Department of General Thoracic and Regenerative Surgery, and Intrathoracic BiotransplantationUniversity Hospital CareggiFlorenceItaly
  3. 3.University College LondonLondonUK
  4. 4.Advanced Center for Translational Regenerative Medicine (ACTREM)ENT (CLINTEC), Karolinska InstitutetHuddinge StockholmSweden

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