Technologies for Detecting Metals in Single Cells

Part of the Metal Ions in Life Sciences book series (MILS, volume 12)

Abstract

In order to fully understand the metallomics of an organism, it is essential to know how much metal is present in each cell and, ideally, to know both the spatial and chemical distributions of each metal (i.e., where within the cell is a metal found, and in what chemical form). No single technique provides all of this information. This chapter reviews the various methods that can be used and the strengths and weaknesses of each.

Keywords

fluorophore laser ablation mass spectrometry nanoprobe PIXE secondary ion SIMS X-ray fluorescence Please cite as: Met. Ions Life Sci. 12 (2013) 15–40 

Abbreviations and Definitions

EDS

electron dispersive spectroscopy

EELS

electron energy loss spectroscopy

EPMA

electron probe microanalysis

ESI

electrospray ionization

FRET

Förster resonance energy transfer

ICP

inductively coupled plasma

IR

infrared

MS

mass spectrometry

MALDI

matrix-assisted laser desorption/ionization

MRI

magnetic resonance imaging

PIXE

proton induced X-ray emission (sometimes particle instead of proton)

SE

secondary electron

SIMS

secondary ion mass spectrometry

SXRF

synchrotron induced X-ray fluorescence

TOF

time-of-flight

XANES

X-ray absorption near edge structure

XAS

X-ray absorption spectroscopy

XFM

X-ray fluorescence microscopy

XRF

X-ray fluorescence

Notes

Acknowledgments

  Some of the measurements described in this chapter were supported by the National Institutes of Health, Grants GM-38047 and GM-70545. The XRF imaging work that was described was all measured at one or more national synchrotron laboratories, much of it at laboratories supported by the US Department of Energy Office of Science.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Departments of Chemistry and BiophysicsUniversity of MichiganAnn ArborUSA

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