Metallomics and the Cell pp 15-40

Part of the Metal Ions in Life Sciences book series (MILS, volume 12)

Technologies for Detecting Metals in Single Cells

Abstract

In order to fully understand the metallomics of an organism, it is essential to know how much metal is present in each cell and, ideally, to know both the spatial and chemical distributions of each metal (i.e., where within the cell is a metal found, and in what chemical form). No single technique provides all of this information. This chapter reviews the various methods that can be used and the strengths and weaknesses of each.

Keywords

fluorophore laser ablation mass spectrometry nanoprobe PIXE secondary ion SIMS X-ray fluorescence Please cite as: Met. Ions Life Sci. 12 (2013) 15–40 

Abbreviations and Definitions

EDS

electron dispersive spectroscopy

EELS

electron energy loss spectroscopy

EPMA

electron probe microanalysis

ESI

electrospray ionization

FRET

Förster resonance energy transfer

ICP

inductively coupled plasma

IR

infrared

MS

mass spectrometry

MALDI

matrix-assisted laser desorption/ionization

MRI

magnetic resonance imaging

PIXE

proton induced X-ray emission (sometimes particle instead of proton)

SE

secondary electron

SIMS

secondary ion mass spectrometry

SXRF

synchrotron induced X-ray fluorescence

TOF

time-of-flight

XANES

X-ray absorption near edge structure

XAS

X-ray absorption spectroscopy

XFM

X-ray fluorescence microscopy

XRF

X-ray fluorescence

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Departments of Chemistry and BiophysicsUniversity of MichiganAnn ArborUSA

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