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Natural and Artificial Proteins Containing Cadmium

  • Anna F. A. PeacockEmail author
  • Vincent L. PecoraroEmail author
Chapter
Part of the Metal Ions in Life Sciences book series (MILS, volume 11)

Abstract

This chapter describes an approach using designed proteins to understand the structure, spectroscopy, and dynamics of proteins that bind Cd(II). We will show that three-stranded coiled coils (3SCCs) based on the parent peptides TRI (Ac-G(LKALEEK)4G-NH2) or GRAND (Ac-G(LKALEEK)5G-NH2) have been essential for understanding how Cd(II) binds to thiolate-rich environments in proteins. Examples are given correlating physical properties such as the binding constants or deprotonation constants relating to structure. We present a scale that relates 113Cd NMR chemical shifts to structures extracted from 111mCd PAC experiments. In addition, we describe motional processes that help transport from the helical interface of proteins into the hydrophobic interior of helical bundles. These studies help clarify the chemistry of Cd(II) in relation to metal-regulated gene expression and detoxification.

Keywords

coiled coil de novo design peptide protein thiol 

Abbreviations and Definitions

&

For the definition of the peptides see Table 1.

Ala

alanine

ALAD

aminolevulinic acid dehydratase

Asp

aspartic acid

CD

circular dichroism

Cys

cysteine

EXAFS

extended X-ray absorption fine structure

Glu

glutamic acid

Gly

glycine

His

histidine

Hfl

hexafluoroleucine

Ile

isoleucine

Leu

leucine

LMCT

ligand-to-metal charge-transfer

Met

methionine

NOESY

nuclear Overhauser effect specroscpy

PAC

perturbed angular correlation

PDB

Protein Data Bank

Pen

penicillamine

NMR

nuclear magnetic resonance

3SCC

three-stranded coiled coil

Ser

serine

Val

valine

Notes

Acknowledgments

A.F.A.P. thanks the University of Birmingham and V.L.P. thanks the University of Michigan and the National Institute of Health for support of this research (R01 ES0 12236).

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© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Department of ChemistryUniversity of MichiganAnn ArborUSA
  2. 2.School of ChemistryUniversity of BirminghamEdgbastonUK

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