Tunable Promoters in Synthetic and Systems Biology

  • Tore Dehli
  • Christian Solem
  • Peter Ruhdal Jensen
Part of the Subcellular Biochemistry book series (SCBI, volume 64)

Abstract

Synthetic and systems biologists need standardized, modular and orthogonal tools yielding predictable functions in vivo. In systems biology such tools are needed to quantitatively analyze the behavior of biological systems while the efficient engineering of artificial gene networks is central in synthetic biology. A number of tools exist to manipulate the steps in between gene sequence and functional protein in living cells, but out of these the most straight-forward approach is to alter the gene expression level by manipulating the promoter sequence. Some of the promoter tuning tools available for accomplishing such altered gene expression levels are discussed here along with examples of their use, and ideas for new tools are described. The road ahead looks very promising for synthetic and systems biologists as tools to achieve just about anything in terms of tuning and timing multiple gene expression levels using libraries of synthetic promoters now exist.

Keywords

Metabolic engineering Standardization, orthogonality and modularity Synthetic and systems biology Synthetic gene networks Synthetic promoter libraries 

Abbreviations

ATc

anhydrotetracycline

DXP

1-deoxy-D-xylulose-5-phosphate

EGFP

enhanced green fluorescent protein

GFP

green fluorescent protein

GOI

gene of interest

gTME

global transcription machinery engineering

GusA

β-glucuronidase

KO

knock-out

MAGE

multiplex automated genome engineering

MCA

metabolic control analysis

ORF

open reading frame

Pfk

phosphofructokinase

PLS-R

partial least squares regression

PSP

promoter strength predictive

RBS

ribosome binding site

SOE PCR

splicing by overlap extension PCR

SPL

synthetic promoter library

TAL

transcription activator-like

TALOR

transcription activator-like orthogonal repressor

yEGFP

yeast enhanced green fluorescent protein

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Tore Dehli
    • 1
  • Christian Solem
    • 1
  • Peter Ruhdal Jensen
    • 1
  1. 1.Center for Systems Microbiology, Department of Systems BiologyTechnical University of DenmarkLyngbyDenmark

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