Tracing Nitrogen Sources and Cycle in Freshwater Through Nitrogen and Oxygen Isotopic Research

  • Zi-Xiang Chen
  • Xue-Bin Yin
  • Guang Liu
  • Gui-Jian Liu
Chapter

Abstract

The technique of using nitrogen and oxygen isotopic compositions to identify nitrogen sources and assess the nitrogen cycle process has been extensively applied in freshwater research in the last few decades. Nitrate pollution in freshwater has caused concern worldwide: if nitrate sources could be identified, this major form of pollution could be combated. Thus, this technique provides much-needed scientific help with the remediation of freshwater. As the method of determining nitrogen and oxygen isotopic ratios has become more precise, many deficiencies in earlier methods of determination have been overcome. Moreover, each kind of nitrate source and some isotopic fractionation processes have also been well studied; all these factors play an important role in research into the use of isotopic tools in tracing nitrate sources. The joint use dual isotopes with water chemistry are indeed providing very detailed and useful information relating to the identification of nitrate sources and the tracking of nitrate transformation in freshwater systems. Recently, some other stable isotopic tools, which are not affected by biogeochemical processes, have been incorporated into dual-isotope research. These tools are better able to respond to the complexities of environments containing multiple potential nitrate sources coexisting in freshwater system, as well as the mixing of point and nonpoint sources in freshwater systems. At the end of this chapter, two actual researches on surface and groundwater were cited as examples for the better comprehension about the dual-isotope work.

Keywords

Isotopic Fractionation Oxygen Isotopic Composition Freshwater System Ammonia Volatilization Synthetic Fertilizer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Heaton THE (1986) Isotopic studies of nitrogen pollution in the hydrosphere and atmosphere: a review. Chem Geol (Isot Geosci Sect) 59:87–102CrossRefGoogle Scholar
  2. 2.
    Li SL, Liu CQ, Li J, Liu XL, Benjamin C, Wang B, Wang FS (2010) Assessment of the sources of nitrate in the Changjiang River, China using a nitrogen and oxygen isotopic approach. Environ Sci Technol 44(5):1573–1578CrossRefGoogle Scholar
  3. 3.
    Wu CC et al (2009) Inter comparison of targeted observation guidance for tropical cyclones in the Northwestern Pacific. Mon Weather Rev 137:2471–2492CrossRefGoogle Scholar
  4. 4.
    Kendall C, Elliott EM, Wankel SD (2007) Tracing anthropogenic inputs of nitrogen to ecosystems. In: Michener R, Lajtha K (eds) Stable isotopes in ecology and environmental science, 2nd edn. Blackwell, Oxford, UK. doi: 10.1002/9780470691854.ch12 Google Scholar
  5. 5.
    Lee KS, Bong YS, Lee DH, Kim YJ, Kim KJ (2008) Tracking the sources of nitrate in the Han River watershed in Korea, using δ15N-NO3 and δ18O-NO3 values. Sci Total Environ 395(2–3):117–124CrossRefGoogle Scholar
  6. 6.
    Kendall C (1998) Tracing sources and cycling of nitrate in catchments. In: Kendall C, McDonnell JJ (eds) Isotope tracers in catchment hydrology. Elsevier, Amsterdam, pp 519–576CrossRefGoogle Scholar
  7. 7.
    Liu CQ, Li SL, Lang YC, Xiao HY (2006) Using delta N-15 and delta O-18 values to identify nitrate sources in karst ground water, Guiyang, Southwest China. Environ Sci Technol 40(22):6928–6933CrossRefGoogle Scholar
  8. 8.
    Silva SR, Lee PB, Ebbert RW et al (2002) Forensic applications of nitrogen and oxygen isotopes of nitrate in an urban environment. Environ Forensic 3:125–130CrossRefGoogle Scholar
  9. 9.
    Seiler RL (2005) Combined use of 15N and 18O of nitrate and 11B to evaluate nitrate contamination in groundwater. Appl Geochem 20:1626–1636CrossRefGoogle Scholar
  10. 10.
    Kendall C, Grim E (1990) Combustion tube method for measurement of nitrogen isotope ratios using calcium oxide for total removal of carbon dioxide and water. Anal Chem 62:526–529CrossRefGoogle Scholar
  11. 11.
    Cook GA, Lauer CM (1968) Oxygen. In: Hampel CA (ed) The encyclopedia of the chemical elements. Reinhold Book Corporation, New York, pp 499–512, LCCN 68-29938Google Scholar
  12. 12.
    Anderson IC, Levine JS (1986) Relative rates of nitric oxide and nitrous oxide production by nitrifiers, denitrifiers, and nitrate respirers. Appl Environ Microbiol 51:938–945Google Scholar
  13. 13.
    Chang CCY, Langston J, Riggs M, Campbell DH, Silva SR, Kendall C (1999) A method for nitrate collection for 15N and18O analysis from waters with low nitrate concentrations. Can J Fish Aquat Sci 56:1856–1864CrossRefGoogle Scholar
  14. 14.
    Silva SR, Kendall C, Wilkison DH, Ziegler AC, Chang CCY, Avanzino RJ (2000) A new method for collection of nitrate from fresh water and the analysis of nitrogen and oxygen isotope ratios. J Hydrol 228:22–36CrossRefGoogle Scholar
  15. 15.
    Fukada T, Hiscock KM, Dennis PF, Grischek T (2003) A dual isotope approach to identify denitrification in ground water at a river bank infiltration site. Water Res 37:3070–3078CrossRefGoogle Scholar
  16. 16.
    Rock L, Ellert BH (2007) Nitrogen-15 and oxygen-18 natural abundance of potassium chloride extractable soil nitrate using the denitrifier method. Soil Sci Soc Am J 71:355–361CrossRefGoogle Scholar
  17. 17.
    Sigman DM, Casciotti KL, Andreani M, Barford C, Galanter M, Böhlke JK (2001) A bacterial method for the nitrogen isotopic analysis of nitrate in seawater and freshwater. Anal Chem 73:4145–4153CrossRefGoogle Scholar
  18. 18.
    Casciotti KL, Sigman DM, Galanter Hastings M, Bohlke JK, Hilkert A (2002) Measurement of the oxygen isotopic composition of nitrate in seawater and freshwater using the denitrifier method. Anal Chem 74:4905–4912CrossRefGoogle Scholar
  19. 19.
    McIlvin MR, Altabet MA (2005) Chemical conversion of nitrate and nitrite to nitrous oxide for nitrogen and oxygen isotopic analysis in freshwater and seawater. Anal Chem 77:5589–5595CrossRefGoogle Scholar
  20. 20.
    Margeson JH, Suggs JC, Midgett MR (1980) Reduction of nitrate to nitrite with cadmium. Anal Chem 52:1955–1957CrossRefGoogle Scholar
  21. 21.
    Wood ED, Armstrong FAJ, Richards FA (1967) Determination of nitrate in seawater by cadmium–copper reduction to nitrite. J Mar Biol Assoc UK 47:23–31CrossRefGoogle Scholar
  22. 22.
    Schilman B, Teplyakov N (2007) Detailed protocol for nitrate chemical reduction to nitrous oxide for delta15N and delta18O analysis of nitrate in fresh and marine waters. Annual report submitted to the Earth Science Research Administration, Ministry of National Infrastructures Jerusalem, December 2007 TR-GSI/15/2007Google Scholar
  23. 23.
    Kohl DH, Shearer GB, Commoner B (1971) Fertilizer nitrogen contribution to nitrate in surface water in a corn belt watershed. Science 174:1331–1334CrossRefGoogle Scholar
  24. 24.
    Elliott EM, Kendall C, Burns DA et al (2006) Nitrate isotopes in precipitation to distinguish NOx sources, atmospheric processes, and source areas in the United States. Eos (Trans. Am. Geophys. Union) 87(36)Google Scholar
  25. 25.
    Hübner H (1986) Isotope effects of nitrogen in the soil and biosphere. In: Fritz P, Fontes JC (eds) Handbook of environmental isotope geochemistry. Elsevier, Amsterdam, pp 361–425Google Scholar
  26. 26.
    Freyer HD (1991) Seasonal variation of 15N/14N ratios in atmospheric nitrate species. Tellus 43B:30–44Google Scholar
  27. 27.
    Amberger A, Schmidt HL (1987) Naturliche Isotopengehalte von Nitat als Indikatoren fur dessen Herkunft. Geochim Cosmochim Acta 51:2699–2705CrossRefGoogle Scholar
  28. 28.
    Durka W, Schulze ED, Gebauer G, Voerkelius S (1994) Effects of forest decline on uptake and leaching of deposited nitrate determined from 15N and 18O measurements. Nature 372:765–767CrossRefGoogle Scholar
  29. 29.
    Wahlen M, Yoshinari T (1985) Oxygen isotope ratios in N2O from different environments. Nature 313:780–782CrossRefGoogle Scholar
  30. 30.
    Flipse WJ, Bonner FT (1985) Nitrogen-isotope ratios of nitrate in ground water under fertilized fields, Long Island, New York. Ground Water 23:59–67CrossRefGoogle Scholar
  31. 31.
    Xue DM, Botte J et al (2009) Present limitations and future prospects of stable isotope methods for nitrate source identification in surface- and groundwater. Water Res 43(5):1159–1170CrossRefGoogle Scholar
  32. 32.
    Fogg GE, Rolston DE, Decker DL, Louie DT, Grismer ME (1998) Spatial variation in nitrogen isotope values beneath nitrate contamination sources. Ground Water 36:418–426CrossRefGoogle Scholar
  33. 33.
    Mayer B, Boyer EW, Goodale C, Jaworski NA, Breemen NV, Howarth RW, Seitzinger S, Billen G, Lajtha K, Nadelhoffer K, Dam DV, Hetling LJ, Nosal M, Paustian K (2002) Sources of nitrate in rivers draining sixteen watersheds in the northeastern U.S.: isotopic constraints. Biogeochemistry 57(58):171–197CrossRefGoogle Scholar
  34. 34.
    Pardo LH, Kendall C, Pett-Ridge J, Chang CCY (2004) Evaluating the source of stream water nitrate using 15N and18O in nitrate in two watersheds in New Hampshire, USA. Hydrol Process 18:2699–2712CrossRefGoogle Scholar
  35. 35.
    Kendall C, Silva SR, Chang CCY, Burns DA, Campbell DH, Shanley JB (1996) Use of the Delta 18-O and Delta 15-N of nitrate to determine sources of nitrate in early spring runoff in forested catchments. In: Isotopes in water resources management. International atomic energy agency symposium vol. 1, Vienna (pp 167–176)Google Scholar
  36. 36.
    Wassenaar LI (1995) Evaluation of the origin and fate of nitrate in the Abbotsford Aquifer using the isotopes of 15N and 18O in NO3. Appl Geochem 10:391–405CrossRefGoogle Scholar
  37. 37.
    Hollocher TC (1984) Source of the oxygen atoms of nitrate in the oxidation of nitrite by Nitrobacter agilis and evidence against a P–O–N anhydride mechanism in oxidative phosphorylation. Arch Biochem Biophys 233:721–727CrossRefGoogle Scholar
  38. 38.
    Kellman LM (2005) A study of tile drain nitrate-delta N-15 values as a tool for assessing nitrate sources in an agricultural region. Nutr Cycle Agroecosyst 71:131–137CrossRefGoogle Scholar
  39. 39.
    Griffiths H (ed) (1998) Stable isotopes: integration of biological, ecological and geochemical processes. Bios Scientific, Herndon, 438 pGoogle Scholar
  40. 40.
    Handley LL, Scrimgeour CM (1997) Terrestrial plant ecology and 15N natural abundance: the present limits to interpretation for uncultivated systems with original data from a Scottish old field. Adv Ecol Res 27:133–212CrossRefGoogle Scholar
  41. 41.
    Högberg P (1997) 15N natural abundance in soil-plant systems. New Phytol 137:179–203CrossRefGoogle Scholar
  42. 42.
    Feigin A, Shearer G, Kohl DH, Commoner B (1974) The amount and nitrogen-15 content of nitrate in soil profiles from two central Illinois fields in a corn-soybean rotation. Soil Sci Soc Am Proc 38:465–471CrossRefGoogle Scholar
  43. 43.
    Létolle R (1980) Nitrogen-15 in the natural environment. In: Fritz P, Fontes JC (eds) Handbook of environmental isotope geochemistry, vol 1. Elsevier, New York, pp 407–433Google Scholar
  44. 44.
    Glibert PM, Capone DG (1993) Mineralization and assimilation in aquatic, sediment, and wetland systems. In: Knowles R, Blackburn TH (eds) Nitrogen isotope techniques. Academic Press, San Diego, California, pp 243–272Google Scholar
  45. 45.
    Powlson DS, Barraclough D (1993) Mineralization and assimilation in soil-plant systems. In: Knowles R, Blackburn TH (eds) Nitrogen isotope techniques. Academic Press, San Diego, California, pp 209–242Google Scholar
  46. 46.
    Mariotti A, Germon JC, Hubert P, Kaiser P, Letolle R, Tardieux A, Tardieux P (1981) Experimental determination of nitrogen kinetic isotope fractionation: some principles: illustration for the denitrification and nitrification processes. Plant Soil 62:423–430CrossRefGoogle Scholar
  47. 47.
    Fogel ML, Cifuentes LA (1993) Isotope fractionation during primary production. In: Engel MH, Macko SA (eds) Organic geochemistry. Plenum Press, New York, pp 73–98CrossRefGoogle Scholar
  48. 48.
    Macko SA, Ostrom NE (1994) Molecular and pollution studies using stable isotope. In: Lajtha K, Michner R (eds) Stable isotopes in ecology and environmental science. Blackwell Scientific, Oxford, UK, pp 45–62Google Scholar
  49. 49.
    Kool DM, Wrage N, Oenema O, Dolfing J, Van Groenigen JW (2007) Oxygen exchange between (de)nitrification intermediates and H2O and its implication for source determination of NO3- and N2O: a review. Rapid Commun Mass Spectrom 21:3569–3578CrossRefGoogle Scholar
  50. 50.
    Andersson KK, Hooper AB (1983) O2 and H2O and each the source of one O in NO2 produced from NH3 by Nitrosomonas: 15N evidence. FEBS Lett 164:236–240CrossRefGoogle Scholar
  51. 51.
    Knowles R (1982) Denitrification. Microbiol Rev 46:43–70Google Scholar
  52. 52.
    Koba K, Tokuchi N, Wada E et al (1997) Intermittent denitrification: the application of a 15N natural abundance method to a forested ecosystem. Geochim Cosmochim Acta 61:5043–5050CrossRefGoogle Scholar
  53. 53.
    Sebilo M, Billen G, Mayer B et al (2006) Assessing nitrification and denitrification in the Seine River and Estuary using chemical and isotopic techniques. Ecosystems 9:564–577CrossRefGoogle Scholar
  54. 54.
    Smith RL, Howes BL, Duff JH (1991) Denitrification in nitrate-contaminated groundwater: occurrence in steep vertical geochemical gradients. Geochim Cosmochim Acta 55:1815–1825CrossRefGoogle Scholar
  55. 55.
    Lehmann MF, Reichert P, Bernasconi SM, Barbieri A, McKenzie A (2003) Modelling nitrogen and oxygen isotope fractionation during denitrification in a lacustrine redox-transition zone. Geochim Cosmochim Acta 67:2529–2542CrossRefGoogle Scholar
  56. 56.
    Mengis M, Schiff SL, Harris M, English MC, Aravena R, Elgood RJ, Maclean A (1999) Multiple geochemical and isotopic approaches for assessing ground water NO3 elimination in a riparian zone. Ground Water 37:448–457CrossRefGoogle Scholar
  57. 57.
    Chen FJ, Jia GD, Chen JY (2009) Nitrate sources and watershed denitrification inferred from nitrate dual isotopes in the Beijing River, south China. Biogeochemistry 94(2):163–174CrossRefGoogle Scholar
  58. 58.
    Mosier AR, Schimel DS (1993) Nitrification and denitrification. In: Knowles R, Blackburn TH (eds) Nitrogen isotope techniques. Academic Press, San Diego, California, pp 181–208Google Scholar
  59. 59.
    Town-Small A, McCarthy MJ, Brandes JA, Yang LY, Zhang L, Gardner WS (2007) Stable isotopic composition of nitrate in Lake Taihu, China, and major inflow rivers. Hydrobiologia 194(3):135–140CrossRefGoogle Scholar
  60. 60.
    Choi WJ, Han GH, Lee SM, Lee GT, Yoon KS, Choi SM, Ro HM (2007) Impact of land-use types on nitrate concentration and δ15N in unconfined ground water in rural areas of Korea. Agric Ecosyst Environ 120:259–268CrossRefGoogle Scholar
  61. 61.
    Voss M, Deutsch B, Elmgren R, Humborg C, Kuuppo P, Pastuszak M, Rolff C, Schulte U (2006) Sources identification of nitrate by means of isotopic tracers in the Baltic Sea catchments. Biogeosciences 3:663–676CrossRefGoogle Scholar
  62. 62.
    Lang YC, Liu CQ, Zhao ZQ, Li SL, Han GL (2006) Geochemistry of surface and ground water in Guiyang city, China: water/rock interaction and pollution in karst hydrological system. Appl Geochem 21:887–903CrossRefGoogle Scholar
  63. 63.
    Deutsch B, Kahle P, Voss M (2006) Assessing the source of nitrate pollution in water using stable N and O isotopes. Agron Sustain Dev 26:263–267CrossRefGoogle Scholar
  64. 64.
    Phillips DL, Koch PL (2002) Incorporating concentration dependence in stable isotope mixing models. Oecologia 130:114–125Google Scholar
  65. 65.
    Moore JW, Semmens BX (2008) Incorporating uncertainty and prior information into stable isotope mixing models. Ecol Lett 11:470–480CrossRefGoogle Scholar
  66. 66.
    Elliott EM, Brush GS (2006) Sedimented organic nitrogen isotopes in fresh water wetlands record long-term changes in watershed nitrogen source and land use. Environ Sci Technol 26(40):2910–2916CrossRefGoogle Scholar
  67. 67.
    Harrington RR, Kennedy BP, Chamberlain CP et al (1998) 15 N enrichment in agricultural catchments: field patterns and applications to tracking Atlantic salmon (Salmo salar). Chem Geol 147:281–294CrossRefGoogle Scholar
  68. 68.
    Kohzu A et al (2008) Use of stable nitrogen isotope signatures of riparian macrophytes as an indicator of anthropogenic N inputs to river ecosystems. Environ Sci Technol 42(21):783–7841CrossRefGoogle Scholar
  69. 69.
    Saurer M, Cherubini P, Ammann M et al (2004) First detection of nitrogen from NOx in tree rings: a 15N/14N study near a motorway. Atmos Environ 38:2779–2787CrossRefGoogle Scholar
  70. 70.
    Aravena R, Robertson WD (1998) Use of multiple isotope tracers to evaluate denitrification in ground water: study of nitrate from a large-flux septic system plume. Ground Water 36:975–982CrossRefGoogle Scholar
  71. 71.
    Bassett RL (1990) A critical evaluation of the available measurements for the stable isotopes of boron. Appl Geochem 5:541–554CrossRefGoogle Scholar
  72. 72.
    Vengosh A, Heumann KG, Juraske S, Kasher R (1994) Boron isotope application for tracing sources of contamination in groundwater. Environ Sci Technol 28:1968–1974CrossRefGoogle Scholar
  73. 73.
    Eisenhut S, Heumann K, Vengosh A (1996) Determination of boron isotopic variations in aquatic systems with negative thermal ionization mass spectrometry as a tracer for anthropogenic influences. Fresenius J Anal Chem 345:903–909Google Scholar
  74. 74.
    Komor SC (1997) Boron contents and isotopic compositions of hog manure, selected fertilizers, and water in Minnesota. J Environ Qual 26:1212–1222CrossRefGoogle Scholar
  75. 75.
    Vitoria L, Otero N, Soler A, Canals A (2004) Fertilizer characterization: isotopic data (N, S, O, C, and Sr). Environ Sci Technol 38(12):3254–3262CrossRefGoogle Scholar
  76. 76.
    Bullen TD, Kendall C (1998) Tracing of weathering reactions and water flowpaths: a multi-isotope approach. In: Kendall C, McDonnell JJ (eds) Isotope tracers in catchment hydrology. Elsevier, Amsterdam, pp 611–646CrossRefGoogle Scholar
  77. 77.
    Qi HP, Coplen TB, Wang QZ, Wang YH (1997) Unnatural isotopic composition of lithium reagents. Anal Chem 69:4076–4078CrossRefGoogle Scholar
  78. 78.
    Bullen TD, Senior LA (1992) Lithogenic Sr and anthropogenic Li in an urbanized stream basin: isotopic tracers of surface water-groundwater interaction. Eos (Trans Am Geophys Union) 73:F130Google Scholar
  79. 79.
    Conley DJ, Paerl HW, Howarth RW, Boesch DF, Seitzinger SP, Havens KE, Lancelot C, Likens GE (2009) Controlling eutrophication: nitrogen and phosphorus. Science 323:1014–1015CrossRefGoogle Scholar
  80. 80.
    Smith VH, Tilman GD, Nekola JC (1999) Eutrophication: impacts of excess nutrient inputs on freshwater, marine, and terrestrial ecosystems. Environ Pollut 100:179–196CrossRefGoogle Scholar
  81. 81.
    Elsbury K, Paytan A, Kendall C, Young MB, McLaughlin K, Rollog M, Watson S (2009) Using oxygen isotopes of phosphate to trace phosphorus sources and cycling in Lake Erie. Environ Sci Technol 43:3108–3114CrossRefGoogle Scholar
  82. 82.
    McLaughlin K, Kendall C, Silva S, Young M, Paytan A (2006) Phosphate oxygen isotope ratios as a tracer for sources and cycling of phosphate in North San Francisco Bay, California. J Geophys Res 111:G03003CrossRefGoogle Scholar
  83. 83.
    McLaughlin K, Cade-Menun BJ, Paytan A (2006) The oxygen isotopic composition of phosphate in Elkhorn Slough, California: a tracer for phosphate sources. Estuar Coast Shelf Sci 70:499–506CrossRefGoogle Scholar
  84. 84.
    Young MB, Mclaughlin K, Kendall C et al (2009) Characterizing the oxygen isotopic composition of phosphate sources to aquatic ecosystems. Environ Sci Technol 43:5190–5196CrossRefGoogle Scholar
  85. 85.
    Paytan A, McLaughlin K (2007) The oceanic phosphorus cycle. Chem Rev 107:563–576CrossRefGoogle Scholar
  86. 86.
    Blake RE, O’Neil JR, Surkov AV (2005) Biogeochemical cycling of phosphorus: Insights from oxygen isotope effects of phosphoenzymes. Am J Sci 305:596–620CrossRefGoogle Scholar
  87. 87.
    Blake RE, O’Neil JR, Garcia GA (1997) Oxygen isotope systematics of biologically mediated reactions of phosphate: I. Microbial degradation of organ phosphorus compounds. Geochim Cosmochim Acta 61:4411–4422CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Zi-Xiang Chen
    • 1
    • 2
  • Xue-Bin Yin
    • 1
    • 2
  • Guang Liu
    • 1
    • 2
  • Gui-Jian Liu
    • 1
  1. 1.School of Earth and Space ScienceUniversity of Science and Technology of China (USTC)HefeiChina
  2. 2.Advanced Lab for Ecological Safety and Human HealthSuzhou Institute of USTCSuzhouChina

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